Laboratory Methods in Enzymology: DNA: Volume 529
Published on 1. November 2013
Book
Hardback
408 pages
978-0-12-418687-3 (ISBN)
Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Dimensions
Height: 229 mm
Width: 152 mm
Weight
770 gr
ISBN-13
978-0-12-418687-3 (9780124186873)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
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Content
Explanatory chapter: PCR Primer design
Explanatory Chapter: How Plasmid Preparation Kits Work
Explanatory Chapter: Introducing Exogenous DNA into cells
Agarose Gel Electrophoresis
Analysis of DNA by Southern Blotting
Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)
Molecular Cloning
Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway (R) Recombination Cloning Technology and Flp-In T-REx (R) lines
Restrictionless cloning
Isolation of plasmid DNA from bacteria
Preparation of Genomic DNA from Bacteria
Preparation of Genomic DNA from Saccharomyces cerevisiae
Isolation of Genomic DNA from Mammalian Cells
Sanger Dideoxy Sequencing of DNA
Preparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platform
Explanatory Chapter: Next Generation Sequencing
Generating mammalian stable cell lines by electroporation
Transient mammalian cell Transfection with Polyethylenimine (PEI)
Site-Directed Mutagenesis
PCR-based random mutagenesis
Megaprimer Method for Mutagenesis of DNA
Explanatory Chapter: Troubleshooting PCR
Explanatory Chapter: Quantitative PCR
General PCR
Colony PCR
Chemical Transformation of Yeast
Transformation of E. coli via electroporation
Transformation of Chemically Competent E. coli
Explanatory Chapter: How Plasmid Preparation Kits Work
Explanatory Chapter: Introducing Exogenous DNA into cells
Agarose Gel Electrophoresis
Analysis of DNA by Southern Blotting
Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)
Molecular Cloning
Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway (R) Recombination Cloning Technology and Flp-In T-REx (R) lines
Restrictionless cloning
Isolation of plasmid DNA from bacteria
Preparation of Genomic DNA from Bacteria
Preparation of Genomic DNA from Saccharomyces cerevisiae
Isolation of Genomic DNA from Mammalian Cells
Sanger Dideoxy Sequencing of DNA
Preparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platform
Explanatory Chapter: Next Generation Sequencing
Generating mammalian stable cell lines by electroporation
Transient mammalian cell Transfection with Polyethylenimine (PEI)
Site-Directed Mutagenesis
PCR-based random mutagenesis
Megaprimer Method for Mutagenesis of DNA
Explanatory Chapter: Troubleshooting PCR
Explanatory Chapter: Quantitative PCR
General PCR
Colony PCR
Chemical Transformation of Yeast
Transformation of E. coli via electroporation
Transformation of Chemically Competent E. coli