
Enzyme Histochemistry
A Laboratory Manual
Springer (Publisher)
1st Edition
Published on 1. June 1979
Book
Paperback/Softback
X, 344 pages
978-3-540-09269-8 (ISBN)
Description
During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.
More details
Language
English
Place of publication
Berlin
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Research
Illustrations
116 s/w Abbildungen
X, 344 p. 116 illus.
Dimensions
Height: 244 mm
Width: 170 mm
Thickness: 20 mm
Weight
615 gr
ISBN-13
978-3-540-09269-8 (9783540092698)
DOI
10.1007/978-3-642-67234-7
Schweitzer Classification
Other editions
Additional editions

E-Book
12/2012
Springer
€96.29
Available for download
Content
A. Introduction.- B. General Considerations.- I. Conditions for the Histochemical Demonstration of Enzymes.- II. Terminology and Classification of Enzymes.- III. Principles of Reactions in Histochemical Methods for the Detection of Enzymes.- IV. Artifacts and Control Reactions.- V. Correlation of Histochemical and Biochemical Findings.- C. Preparation of Tissues.- I. Tissue Sampling.- II. Further Treatment.- III. Incubation.- D. Detection Methods.- I. Instructions.- II. Hydrolases.- III. Transferases.- IV. Lyases.- V. Oxidoreductases.- E. Solutions and Buffers.- F. List of Firms.- G. References.- H. Subject Index and Source List.