High Performance Liquid Chromatography

HPLC is the most powerful of all chromatographic techniques but because of its sophistication, it requires both extensive experience and good theoretical knowledge. The book provides practical experience in the use of basic HPLC equipment, demonstrates the various parameters that control HPLC equipment, demonstrates the various parameters that control HPLC separations, shows the use of the technique for separation and quantitative analysis and illustrates some of the important principles from the theory part of the unit. All the background to the technique, detail on available instrumentation and on appreciation of results interpretation and the effect of varying instrumental parameters are covered.