Handbook of Practical Immunohistochemistry
Description
In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes.
Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence.
Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
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Content
1. Quality management and regulation
Jeffrey Prichard
2. Technique and troubleshooting of antibody testing
Fan Lin and Jianhui Shi
3. Overview of automated immunohistochemistry
Jeff Prichard, Angela Bitting and Joe Myers
4. Automated staining - Dako Perspective
Ole F. Rasmussen and Andreas Schønau
5. Automated staining - Ventana Perspective
Angela Sattler
6. Tissue microarray
Myra Wilkerson and Erin Powell
7. Unknown primary/undifferentiated neoplasms in surgical and cytologic specimens
Fan Lin and Haiyan Liu
8. Exfoliative cytopathology
Steven Meschter and Jan F. Silverman
9. Predictive markers of breast cancer: ER, PR and Her-2/neu
Jeffrey Prichard
10. Central and peripheral nerve system tumors
Hueizhi (Hope) Wu, Conrad Schuerch, and Douglas C. Miller
11. Thyroid and parathyroid gland
Haiyan Liu, Fan Lin and Ronald A. DeLellis
12. Adrenal gland
Hanna Kaspar
13. Salivary gland and other head and neck structures
Conrad Schuerch
14. Lung, pleura, and mediastinum
Kai Zhang and Phillip Cagle
15. Breast
Haiyan Liu
16. Uterus
Hanna Kaspar
17. Ovary
Jeffrey Prichard, Haiyan Liu and Myra Wilkerson
18. Prostate gland
Haiyan Liu, Fan Lin and Qihui (Jim) Zhai
19. Urinary bladder
Myra Wilkerson
20. Kidney
Fan Lin and Ximing J. Yang
21. Testis and paratesticular tissues
Myra Wilkerson
22. Pancreas and ampullae
Fan Lin and Hanlin L. Wang
23. Liver, bile ducts and gallbladder
Jeffrey Prichard and Fan Lin
24. Upper gastrointestinal tract
Jinhong Li and Fan Lin
25. Lower gastrointestinal tract and microsatellite instability (MSI)
Jinhong Li and Fan Lin
26. Soft tissue and bone tumors
Shaobo Zhu and Markku Miettinen
27. Lymph node Xiaohong (Mary) Zhang and Nadine S. Aguilera
28. Bone Marrow
R. Patrick Dorion and Xiaohong (Mary) Zhang
29. Infectious diseases
Dirk M. Elston, Lawrence E. Gibson and Heinz Kutzner
30. Skin
Tammie Ferringer
31. Application of Direct Immunofluorescence for skin and mucosal biopsies
William B. Tyler
32. In situ hybridization in surgical and cytologic specimens
Hong Yin and Barbara Paynton
33. Appendices - Antibody information and protocols (Appendix I using Dako System; Appendix II using Ventana System)
Fan Lin, Jianhui Shi, Angela Bitting, and Jeffrey Prichard