Antisense Technology
Conrad Lichtenstein(Author)
Oxford University Press
Published on 1. September 1997
Book
Hardback
325 pages
978-0-19-963584-9 (ISBN)
Description
Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologies are then described, with chapters on antisense techniques in IDictyostelium , plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo . This book is intended for graduate and post-doctoral researchers and academic staff.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
23 halftones, 56 line figures, bibliography
ISBN-13
978-0-19-963584-9 (9780199635849)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
1: Nucleic acid structures. 2: Evaluation of antisense effects. 3: Selecting, preparing, and handling antisense oligodeoxyribonucleotides. 4: In vitro RNAs. 5: Catalytic antisense RNA based on hammerhead ribozymes. 6: 2-5 A-Antisense chimaeras for targeted degradation of RNA. 7: In vitro applications of antisense RNA and DNA. 8: Antisense applications in Dictyostelium: a lower eukaryotic model system. 9: Applications of antisense technology in plants. 10: Antisense molecules and ribozymes: medical applications. 11: Non-antisense effects of oligodeoxynucleotides. 12: Antisense rescue. 13: Shotgun antisense mutants. 14: Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies