Differential Display Methods and Protocols
2nd Edition
Published on 21. October 2005
Book
Hardback
XIV, 320 pages
978-1-58829-338-1 (ISBN)
Description
Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an "open"-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms-from bacteria, fungi, insects, fish, plants, to mammals-even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious "cottage-industry" academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.
Reviews / Votes
"The variety of applications...is so broad that almost every newcomer...can use these protocols as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field." -Trends in BiotechnologyFrom Reviews of the First Edition...
"...really helpful for all geneticists and cellular and molecular biologists...a precious book which should be in all hands!" -Cellular and Molecular Biology
"...For any would-be gene hunter thinking of or already practicing differential display, this book will provide an invaluable and long overdue guide to the technique." -Molecular Medicine
"....an ideal resource for any research group interested in using DD." -Microbiology Today
"...highly recommend[ed]...for purchase by everyone interested in the comparative gene expression,, and especially to newcomers to the field." -Trends in Biotechnology
More details
Series
Edition
Second Edition 2006
Language
English
Place of publication
Totowa
United States
Target group
Professional and scholarly
Research
Edition type
Revised edition
Illustrations
131 s/w Abbildungen, 1 farbige Abbildung
XIV, 320 p. 132 illus., 1 illus. in color.
Dimensions
Height: 241 mm
Width: 160 mm
Thickness: 26 mm
Weight
732 gr
ISBN-13
978-1-58829-338-1 (9781588293381)
DOI
10.1385/1592599680
Schweitzer Classification
Other editions
Additional editions
Peng Liang | Jonathan Meade | Arthur B. Pardee
Differential Display Methods and Protocols
Book
12/2010
2nd Edition
Humana Press Inc.
€106.99
Shipment within 15-20 days
Previous edition
Book
12/2009
Humana Press Inc.
€94.09
Article exhausted; check for reprint
Content
Methodologies.- Global Analysis of Gene Expression by Differential Display.- Automation of Fluorescent Differential Display With Digital Readout.- Ordered Differential Display.- GeneCalling®.- High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR.- Vertical Arrays.- Automated Pattern Ranking in Differential Display Data Analysis.- Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs.- Applications.- Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma.- Identification of Disease Markers by Differential Display.- Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display.- Identification of p53-Regulated Genes by the Method of Differential Display.- Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes.- Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display.- Functional Analysis of Nocturnin.- Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display.- Identification of Target Genes of a Yeast Transcriptional Repressor.- Detection of an mRNA Polymorphism by Differential Display.- Silencing in Yeast.- Identification of mRNA Bound to RNA Binding Proteins by Differential Display.