Clinical Microbiology Procedures Handbook, 5th Edi tion Multi-Volume
Published on 29. May 2023
Book
Hardback
3792 pages
978-1-68367-398-9 (ISBN)
Description
Gold Standard consensus-based procedures from the experts.
The Clinical Microbiology Procedures Handbook, 5th edition, provides those engaged in microbial analysis of clinical specimens with procedures for the detection, identification, and characterization of microorganisms involved in human infections. This unique and valuable collection of step-by-step descriptions of the numerous testing modalities used in the clinical microbiology laboratory was written and edited by highly knowledgeable laboratorians. The 5th edition features two new sections, one on blood cultures and one on MALDI-TOF MS, and the sections on molecular diagnostics, virology, and serology were extensively revised and updated. Presented over multiple volumes, this handbook enables laboratory staff to perform all analyses, including appropriate quality control recommendations, from the receipt of the specimen through processing, testing, interpretation, presentation of the final report, and subsequent consultation.
More details
Edition
5th Edition
Language
English
Place of publication
United States
Target group
Professional and scholarly
Dimensions
Height: 296 mm
Width: 242 mm
Thickness: 189 mm
Weight
9796 gr
ISBN-13
978-1-68367-398-9 (9781683673989)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Amy L. Leber | Carey-Ann D. Burnham
Clinical Microbiology Procedures Handbook, Multi-Volume
E-Book
11/2024
5th Edition
Wiley
€380.99
Available for download
Amy L. Leber | Carey-Ann D. Burnham
Clinical Microbiology Procedures Handbook, Multi-Volume
E-Book
11/2024
5th Edition
Wiley
€380.99
Available for download
Previous edition
Book
05/2016
4th Edition
American Society for Microbiology
Unfortunately, price unknown
Article exhausted; check for reprint
Content
SECTION 1 Procedure Coding, Reimbursement, and Billing Compliance
SECTION EDITORS: Vickie Baselski and Alice Weissfeld
1.1. Introduction
Vickie Baselski and Alice Weissfeld.....................................................................................................1.1.1
1.2. Procedure Coding, Reimbursement, and Billing Compliance
Vickie Baselski and Alice Weissfeld.................................................................................................1.2.1.1
1.2.1 Procedure Coding....................................................................................................................1.2.1.1
1.2.2 Procedure BillingB...............................................................................................................1.2.2.1
1.2.3 Coverage of Laboratory Services..........................................................................................1.2.3.1
1.2.4 Billing Compliance Programs.................................................................................................1.2.4.1
SECTION 2
Specimen Collection, Transport, and Acceptability
SECTION EDITORS: Andrea Linscott and Huanyu Wang
2.1. Collection, Transport, and Manipulation of Clinical Specimens and Initial Laboratory Concerns
Andrea Linscott...........2.1.1
Table 2.1-1. "Rule-Out" Clinical Impressions and Potential Etiological Agents...........2.1.6
Table 2.1-2. General Principles for Specimen Collection...........2.1.17
Table 2.1-3. Common Transport Media...........2.1.17
Table 2.1-4. Collection of Specimens for Bacteriological Analysis...........2.1.18
Table 2.1-5. Rejection Criteria for Microbiological Specimens...........2.1.25
Table 2.1-6. Collection of Specimens to Detect Infrequently Encountered Organisms...........2.1.28
Table 2.1-7. Collection of Specimens for Virological Analysis...2.1.30
Table 2.1-8. Laboratory Approaches to Suspected Fungal Infections...........2.1.34
Table 2.1-9. Collection of Specimen to Detect Parasites...........2.1.35
Table 2.1-10. Specimen Processing Triage...........2.1.37
Table 2.1-11. Procedure for Processing Clinical Specimens in Microbiology...........2.1.37
Table 2.1-12. Critical in Microbiology...........2.1.39
Table 2.1-13. Alert Request...........2.1.39
SECTION 3 Aerobic Bacteriology
SECTION EDITOR: Amanda T. Harrington and Melanie L. Yarbrough
3.1. Introduction
Amanda T. Harrington and Melanie L. Yarbrough......................................................................3.1.1
3.2. Staining Procedures
Steven Dallas and Amanda T. Harrington...................................................................................3.2.1.1
3.2.1. Gram Stain...........................................................................................................................3.2.1.1
Appendix 3.2.1-1. Preparation of Gram Stain Reagents......................................3.2.1.X
Appendix 3.2.1-2. Rejection Criteria for Sputum
Culture...........................................................................................................3.2.1.X
Appendix 3.2.1-3. Reporting Gram-Stained Vaginal Smears To Diagnose Bacterial
Vaginosis and Vaginitis...................................................................................3.2.1.X
3.2.2. Acridine Orange Stain ...................................................................................................3.2.2.1
Appendix 3.2.2-1. Preparation of Acridine Orange Stain...........................................3.2.2.X
3.2.3. Vaginal Wet Mount............................................................................................................3.2.3.1
3.2.4. Wet Mount for Detection of Leukocytes and Microorganisms ..................................3.2.3.1
3.3. Processing, Isolation, Detection, and Interpretation of Aerobic Bacteriology Cultures
Amanda T. Harrington and Melanie L. Yarbrough ..................................................................3.3.1.1
3.3.1. Processing of Specimens for Aerobic Bacteriology ................................3.3.1.1
3.3.2. Interpretation and Rapid Identification of Bacterial Growth on Primary Culture Media...3.3.2.1
3.4. Body Fluid Cultures (Excluding Blood, Cerebrospinal Fluid, and Urine)
Nicholas Moore...........................................................................................................................3.4.1
3.5. Cerebrospinal Fluid Cultures
Rangaraj Selvarangan .................................................................................................................3.7.1
3.6 Medical Devices: Pre- and Postimplant testing..............................................................................3.6.1
3.7. Fecal and Other Gastrointestinal Cultures and Toxin Assays
Dylan R. Pillai and Thomas Griener..............................................................................................3.7.1.1
3.8.1. Fecal Culture for Aerobic Pathogens of Gastroenteritis ...........................................3.8.1.1
Appendix 3.7.1-1. Detection of Somatic O Antigen Serogroups of Bacteria...........3.8.1.X
Appendix 3.7.1-2. Detection of Escherichia coli O157 by Latex
Agglutination.......................................................................................................3.8.1.X
Appendix 3.7.1-3. Detection of Shiga Toxin by Immunochromatographic Assay...3.7.1.X
3.7.2. Fecal Culture for Campylobacter and Related Organisms ...........................................3.7.2.1
3.7.3. Helicobacter pylori Cultures ...........................................................................................3.7.3.1
Appendix 3.8.4-1. Helicobacter pylori Antigen Assay (HpSA)...... .............................3.7.3.X
3.7.4. Quantitative Culture of Small-Bowel Contents...........................................................3.7.4.1
3.8. Genital Cultures
Laura Filkins and Omai Garner.......................................................................................................3.8.1.1
3.8.1. Guidelines for Performance of Genital Cultures
3.8.2. Group B Streptococcus Cultures ......................................................................................3.8.2.1
3.8.3. Neisseria gonorrhoeae Cultures .....................................................................................3.8.3.1
3.8.4. Haemophilus ducreyi Cultures ........................................................................................3.8.4.1
3.9. Ocular Cultures
Eileen Burd......................................................................................................................................3.9.1
3.10. Respiratory Tract Cultures..................................................................................................3.10.1.1
3.10.1. Guidelines for Performance of Respiratory Tract Cultures * Kevin Alby and Jason Smedberg.......................................................................................3.10.1.1
3.10.2. Lower Respiratory Tract Cultures * Kevin Alby and Jason Smedberg.............3.10.2.1
Appendix 3.11.2-1. Quantitative Culture of Protected Specimen Brush and
Bronchoalveolar Lavage Fluid Specimens...................................................3.10.2.X
3.10.3. Respiratory Cultures from Cystic Fibrosis Patients * Kevin Alby and Jason Smedberg .....................................................................................................................3.10.3.1
3.10.4. Legionella Cultures and Urinary Antigen Testing* Diane SJ Lindsay and Maria L. Ricci................................................................................................................................3.10.4.1
Appendix 3.10.4-1. Urinary Antigen Testing...........................................................3.10.4.X
3.10.5. Ear Cultures * Melanie L. Yarbrough and Carey-Ann D. Burnham.......................3.10.5.1
3.10.6. Bordetella Cultures * Amy L. Leber ......................................................................3.10.6.1
3.10.7. Culture of Corynebacterium diphtheriae and Other Diphtheria Toxin-Producing Bacterial Species * Allen C. Bateman........................................................................3.10.7.1
3.10.8. Throat Culture and Nonculture Tests for Pharyngitis * Mark D. Gonzalez...........3.10.8.1
Appendix 3.11.8-1. Beta-Hemolytic Streptococcal Latex Agglutination Method..3.10.8.X
3.10.9. Nasal Sinus Cultures * Morgan A. Pence...................................................................3.10.9.1
3.11. Urine Cultures
Melanie L. Yarbrough......................................................................................................................3.11.1
3.11.1. Urine Cultures..................................................................................................................3.11.1.1
Appendix 3.11.1-1. Validation of Urine Inoculation Methods..................................3.11.1.X
3.11.2. Stone Cultures...............................................................................................................3.11.2.1
3.12. Wound Cultures
Alexandra Bryson and Christopher Doern...............................................................................3.12.1.1
3.12.1. Wound/Abscess and Soft Tissue Cultures ...............................................................3.12.1.1
3.12.2. Quantitative Cultures of Wound Tissues .................................................................3.12.2.1
3.13. Leptospira Culture
Renee L. Galloway.......................................................................................................................3.13.1
Appendix 3.13-1. Summary of Diagnostic Tests for Leptospirosis.........................3.13.X
3.14. Detection of Human Mycoplasmas and Ureaplasmas from Clinical Specimens by Culture and PCR
3.14.1. Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma Cultures. * Cécile Bébéar and Ken B. Waites ..................................................................................3.14.1.1
Appendix 3.14.1-1. Medium Formulations for Cultivation of Mycoplasmas and Ureaplasmas from Humans.................................................................................3.14.1.X
3.14.2. Antimicrobial Susceptibility Testing of Mycoplasmas and Ureaplasmas. * Cécile Bébéar and Ken B. Waites ...............................................................................................3.14.2.1
3.14.3. Detection of Mycoplasma pneumoniae by nucleic acid amplification tests. * Cécile Bébéar .......................................................................................................................3.14.3.1
3.14.4. Detection of Mycoplasma genitalium, Mycoplasma hominis and Ureaplasma species by nucleic acid amplification tests. * Cécile Bébéar ...........3.14.4.1
3.15. Bartonella Cultures
Jill Clarridge and Amanda T. Harrington.........................................................................3.15.1
3.16. Guidelines for Biochemical Identification of Aerobic Bacteria
James J. Dunn.......................................................................................................................3.16.1
3.17. Biochemical Tests for the Identification of Aerobic Bacteria
Richard Davis and Marie Pezzlo...........................................................................3.17.1.1
3.17.1. Acetamide Utilization Test (Acetamide Agar) .......................................................17.1.1
3.17.2. Acetate Utilization Test - (Acetate Differential Agar) .......................................3.17.2.1
3.17.3. ALA (delta-Aminolevulinic Acid) Test for Porphyrin Synthesis - Tube or Disk Test ..3.17.3.1
3.17.4. Antimicrobial Disk Tests for Identification ........3.17.4.1
3.17.5. Bile-Esculin and Esculin Tests .................................................................3.17.5.1
3.17.6. Bile Solubility Test ............................................................................................3.17.6.1
3.17.7. Butyrate Esterase Test.........................................................................................3.17.7.1
3.17.8. CAMP Factor Tests (Standard/ Rapid, Reverse and Inhibition) .........3.17.8.1
3.17.9. Carbohydrate Utilization Tests .................................................................3.17.9.1
3.17.10. Catalase Test ............................................................................................................3.17.10.1
3.17.11. Cetrimide Test.........................................................................................3.17.11.1
3.17.12. Citrate Utilization Test (Simmons) .................................................................3.17.12.1
3.17.13. Coagulase Test--Protein A/Clumping Factor Agglutination Method.....3.17.13.1
3.17.14. Coagulase Test--Rabbit Plasma Method ......................................3.17.14.1
3.17.15. Decarboxylase-Dihydrolase Tests..............................................................3.17.15.1
3.17.16. DNase Test-Rapid Thermonuclease Test ..............................................................3.17.16.1
3.17.17. Fluorescent-Pigment Agars for Pseudomonas Identification.............................3.17.17.1
3.17.18. Gelatin Liquefaction.................................................................3.17.18.1
3.17.19. Gram Reaction Enzymatic Test..............................................................3.17.19.1
3.17.20. Hippurate Hydrolysis Rapid Test .................................................................3.17.20.1
3.17.21. Hydrogen Sulfide Production .................................................................3.17.21.1
3.17.22. Indole Test ............................................................................................3.17.22.1
Appendix 3.17.22-1. Reagent Preparation......................................3.17.22.X
3.17.23. Indoxyl Acetate Disk Test.................................................................3.17.23.1
3.17.24. Kligler's Iron Agar Test and Triple Sugar Iron Agar Test.. .............................3.17.24.1
3.17.25. LAP (Leucine Aminopeptidase) Test .................................................................3.17.25.1
3.17.26. Lecithinase and Lipase Detection .................................................................3.17.26.1
Appendix 3.17.26-1. Preparation of Egg Yolk Agar Medium...........3.17.26.X
3.17.27. Lipophilism Test for Corynebacterium ..............................................................3.17.27.1
3.17.28. Malonate Test ............................................................................................3.17.28.1
3.17.29. MGP (Methyl Glucopyranoside) Test .................................................................3.17.29.1
3.17.30. Motility Tests ............................................................................................3.17.30.1
3.17.31. MRS Broth ............................................................................................3.17.31.1
3.17.32. MR-VP (Methyl Red-Voges-Proskauer) Tests ......................................3.17.32.1
3.17.33. MUG (4-Methylumbelliferyl-ß-D-Glucuronide) Test ......................................3.17.33.1
3.17.34. Nitrate/Nitrite Reduction Test .................................................................3.17.34.1
3.17.35. O/129 Disk Susceptibility Testing for Vibrio and Aeromonas spp. .....3.17.35.1
3.17.36. ONPG (o-Nitrophenyl-ß-D-Galactopyranoside) Test ......................................3.17.36.1
3.17.37. Optochin Susceptibility Test .................................................................3.17.37.1
3.17.38. Oxidase Test ............................................................................................3.17.38.1
3.17.39. Phenylalanine Deaminase Test .................................................................3.17.39.1
3.17.40. PYR (L-Pyrrolidonyl-ß-Naphthylamide) Test ......................................3.17.40.1
3.17.41. Quellung Reaction for Streptococcus pneumoniae (Neufeld test) ..................3.17.41.1
3.17.42. 6.5% Salt and Temperature Tolerance Test ......................................3.17.42.1
3.17.43. Satellite Test ............................................................................................3.17.43.1
3.17.44. SPS (Sodium Polyanethol Sulfonate) Disk Test ......................................3.17.44.1
3.17.45. Starch Hydrolysis Test ............................................................................................3.17.45.1
3.17.46. Urease Test ............................................................................................3.17.46.1
3.18. Identification of Gram-Positive Bacteria
Kathryn Bernard..................................................................................................................3.18.1
3.19. Identification of Gram-Negative Bacteria * Erik Munson and Raymon Podzoeaki...........3.19.1
SECTION 4 Anaerobic Bacteriology
SECTION EDITOR: Audrey N. Schuetz
4.1. Introduction
Audrey N. Schuetz..........................................................4.1.1
4.2 Taxonomy Updates for Anaerobes
Samantha K. Shannon and Morgan A. Pence....4.2.1
4.3. Specimen Selection, Collection, and Transport for Anaerobic Culture
Melphine M. Harriott and Kristen L. Brown.....4.3.1
4.4. Culture Media for Anaerobes
Jennifer Dien Bard, Sarah Copsey-Mawer, and Mimi Precit...................................................4.4.1
4.4.1. Primary Culture Media for Anaerobes.......................................4.4.1.1
Appendix 4.3.1-1. Formulas of Media for Anaerobes
4.4.2. Secondary Culture Media for Anaerobes......................................4.4.2.1
4.5. Processing Specimens for Anaerobic Culture
Trefor E. Morris........................................................4.5.1
4.6. Incubation Techniques for Anaerobic Bacteriology Specimens
Trefor E. Morris.......................................................4.6.1
4.7. Examination of Primary Culture Plates for Anaerobic Bacteria
Trefor E. Morris.....................................................4.7.1
4.8. A Practical Guide to the Workup of Anaerobic Cultures
Samantha K. Shannon and Morgan A. Pence....... 4.8.1
4.9. Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes
Andrew Clark and Margaret Ordonez Smith de Daines.........4.9.1.1
4.9.1. Introduction ................................................................. 4.9.1.1
4.9.2. Indole Test ........................................................... 4.9.2.1
4.9.3. Nitrate Disk Reduction Test ............................................ 4.9.3.1
4.9.4. Catalase Test ................................................................ 4.9.4.1
4.9.5. Identification by Using Special-Potency Disks .................... 4.9.5.1
4.9.6. Sodium Polyanethol Sulfonate Disk for Differentiation of Anaerobic Cocci... 4.9.6.1
4.9.7. Bile Test/Bacteroides Bile Esculin Agar for Differentiation of Anaerobic Gram Negative
Rods ................4.9.7.1
4.9.8. Fluorescence ................................................................ 4.9.8.1
4.9.9. Lipase Test .................................................................. 4.9.9.1
4.9.10. Lecithinase Test .........................................................4.9.10.1
4.9.11. Pigment Production ....................................................4.9.11.1
4.9.12. Urease Test ...............................................................4.9.12.1
4.9.13. Gelatinase Production ................................................4.9.13.1
4.9.14 Alkaline Phosphatase ..................................................... 4.9.14.1
4.9.15. Glutamic Acid Decarboxylase ......................................... 4.9.15.1
4.9.16. L-Alanyl-Alanylaminopeptidase ........................................ 4.9.16.1
4.9.17. L-Proline-Aminopeptidase ............................................... 4.9.17.1
4.9.18. 4-Methylumbelliferone Derivative Substrates ..................... 4.9.18.1
4.9.19. Combination Enzymatic Tablets for Nitrophenol, Aminopeptidase, and
Methylumbelliferyl Substrates ................ 4.9.19.1
4.10. Commercial Kit and Rapid Enzymatic Systems for the Identification of Anaerobes
Frances Valencia-Shelton and Jennifer Dien Bard ......4.10.1
4.11. Storage and Stocking of Anaerobes
Frances Valencia-Shelton and Kristen L. Brown.....................4.11.1
4.12. Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight for the Identification of
Anaerobic Bacteria
Ashley L. Paulick and Sarah Copsey-Mawer.................... 4.12.1
4.13. Anaerobic Gram-Negative Bacilli
Rosemary C. She and Dierdre L. Church.............................................. 4.13.1
4.14. Anaerobic Gram-Positive Bacilli
Kathryn Bernard and Camille Hamula............................................... 4.14.1
4.15. Anaerobic Cocci
Rosemary C. She and Deirdre L. Church.................................................................. 4.15.1
4.16. Clostridioides (Clostridium) difficile as a Pathogen Involved in Antimicrobial Agent-Associated Diarrhea, Colitis, and Pseudomembranous Colitis
Karen C. Carroll and Ashley L. Paulick................................................ 4.16.1
Section 5: Blood Culture
5.1 Introduction Amanda Harrington
5.2 Preanalytic Considerations and Laboratory Processing of Blood Culture Amanda Harrington and Robert Tibbetts
5.3 Continuous Monitoring Blood Culture Systemsf or Detection of Aerobic and Anaerobic Bacteria Evann Hilt Evann Hilt and Sophonie Jean
5.4 Lysis Centrifugation Erin McElvania
5.5 Continuous Monitoring Blood Culture Systems for Detection of Acid-Fast Bacilli and Fungi Bill Lainhart and Paul Leuthy
5.6 Quality Control and Quality Assurance Raquel Martinez
5.7 Culture Methods for Detection of Aerobic and Anaerobic Bacteria from Positive Blood Cultures Evann Hilt and Sophonie Jean
5.8 Culture Methods for Detection of Acid-fast Bacilli and Fungi from Positive Blood Culture Bill Lainhart and Paul Leuthy
5.9 Post Analytical Considerations for Blood Culture Joan-Miquel Balada-Llasat and Kamal Kamboj
5.10. Supplementation of Positive Blood Culutres with Chromogenic Media Matthew Faron
5.11 Specialized Processing of Blood Robin Chamberland
5.11.1 Transfusion Reactions Robin Chamberland
5.11.2 "Autopsy Blood Cultures " Robin Chamberland
5.11.3 Special Media and Stains for Fastidious and Infrequently Encountered Organism Robin Chamberland
5.12 Catheter Tip Cultures Steve Miller
5.13 Multi-Plex Molecular Panels for Positive Blood Cultures Rosemary She and Tam Van
5.14 Broad Range PCR and Emerging Technologies for the Detection of Microorganisms from Blood Specimens Dan Greene
5.15 Phenotypic Antimicrobial Susceptibility Testing for Positive Blood Culture April Abbott and Shelley Campeau may not be included in print
Section 6: MALDI-TOF
6.1 Introduction Erin McElvania
6.2 MALDI-TOF) for Bacterial and Yeast Identification Margaret V. Powers-Fletcher and Mark Fisher
6.3 High-Consequence Bacterial Pathogens (BT Agents) Anthony Tran
6.4 Nocardia and Mycobacteria Adrian Zelazny and Joan Miquel Balada-Llasat
6.5 Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) for Filamentous Fungi Identification Kimberly Hanson*, Juliana Sobczyk, and Emily Snavely
6.6 MALDI-TOF MS for Identification of Bacteria and Yeast from Positive Blood Culture Broth Erin McElvania
SECTION 7 Antimicrobial Susceptibility Testing
SECTION EDITORS: Romney M. Humphries and Patricia J. Simner
7.1. Introduction
Romney M. Humphries and Patricia J. Simner
7.1.1. Types of Antimicrobial Susceptibility Tests ...................7.1.1.1
Appendix 7.1.1-1. Surrogate and equivalent agent tests for commonly isolated bacteria
that grow aerobically .............7.1.1.X
7.1.2. Selecting and Applying Clinical Breakpoints ..................7.1.2.1
ROUTINE TESTS PERFORMED FOR AST
7.2. Disk Diffusion Testing
Eric M. Ransom, Carey-Ann D. Burnham, and Erika Matuschek...................................................................7.2.1
Appendix 7.2-1. Quality Control: CLSI .........................7.2.1.X
Appendix 7.2-2. Quality Control: EUCAST ...................7.2.1.X
Appendix 7.2-3. Where to find CLSI and EUCAST disk diffusion criteria for various organisms............................7.2.1.X
Appendix 5.1-4. Zone reading ...................7.2.1.X
7.3. Disk Diffusion from Positive Blood Culture
Gunnar Kahlmeter and Shelley Campeau .......7.3.1
Appendix 7.3-1. Procedures for CLIS and EUCAST disk diffusion from positive blood
cultures ......7.3.X
7.4. Broth Microdilution MIC Test
7.4.1. Broth Microdilution MIC Test* Dulini Gamage and Sukantha Chandrasekaran....................7.4.1.1
Appendix 7.4.1-1. Reference list for performing broth microdilution MIC tests.....................................5.2.1.X
Appendix 7.4.1-2. Broth microdilution QC log sheet.....................................5.2.1.X
Appendix 5.2.1-3. Haemophilus influenzae and Haemophilus parainfluenzae
.....................................5.2.1.X
Appendix 7.4.1-4. Streptococcus pneumoniae and Streptococcus spp. (beta hemolytic group viridans group.......5.2.1.X
Appendix 7.4.1-5. Neisseria meningitidis ............5.2.1.X.
Appendix 7.4.1-6. Breakpoint MIC panels ...........5.2.1.X
Appendix 7.4.1-7. Example of a portion of a MIC breakpoint panel....5.2.1.X
7.4.2. Broth Microdilution MIC Test for Anaerobic Bacteria* Darcie Carpenter............................................7.4.2.1
Appendix 7.4.2-1. Preparation of Media and Reagents ......7.4.2.X
Appendix 7.4.2-2. Anaerobe broth microdilution QC .........7.4.2.X
7.5. Gradient Diffusion Tests
Hari P. Dwivedi, Fabio Brocco, Erika Matuschek ..........................................................7.5.1
Appendix 7.5-1. Gradient test QC table ...............7.5.X
Appendix 7.5-2. Reference list for performing gradient diffusion tests ......7.5.X
Appendix 7.5-3. Photographic reading guide for bacteriostatic vs. bactericidal agents
...7.5.X
7.6. Agar Dilution MIC Test
Samir N. Patel.........................7.5.1
7.6.1. Agar Dilution MIC Test for Aerobic Bacteria...................................................7.6.1.1
Appendix 7.6.1-1. Neisseria meningitidis ...................7.6.1.X
Appendix 7.6.1-2. Neisseria gonorrhoeae ..................7.6.1.X
Appendix 7.6.1-3. Helicobacter pylori.........................7.6.1.X
Appendix 7.6.1-4. Reference guide for performing agar dilution MIC tests....7.6.1.X
Appendix 7.6.1-5. Preparation of MHA deeps............................................7.6.1.X
Appendix 7.6.1-6. Volumes of components required for preparation of agar dilution
plates when using round or square petri plates.................7.6.1.X
Appendix 7.6.1-7. Agar dilution QC. ......................7.6.1.X
Appendix 7.6.1-8. Timetable for agar dilution susceptibility testing of aerobic bacteria
......7.6.1.X
Appendix 7.6.1-9. Preparation of antimicrobial dilutions from stock solutions....7.6.1.X
Appendix 7.6.1-10. Agar dilution MIC worksheet..................................7.6.1.X
7.6.2. Agar Dilution MIC Test for Anaerobic Bacteria.............................................7.6.1.1
Appendix 7.6.2-1. Preparation of media and reagents....7.6.2.X
Appendix 7.6.2-2. Agar dilution QC......................7.6.2.X
Appendix 7.6.2-3. Timetable for agar dilution susceptibility testing of anaerobic
bacteria............7.6.2.X
Appendix 7.6.2-4. Preparation of antimicrobial dilutions from stock solutions.....7.6.2.X
Appendix 7.6.2-5. Volumes of components required for preparation of agar dilution
Plates when using round of square petri plates......7.6.2.X
Appendix 7.6.2-6. Agar dilution MIC worksheet................7.6.2.X
PHENOTYPIC DETECTION OF RESISTANCE MECHANISMS --GENERAL
7.7. Beta-lactamas