
RNAi
Martin Latterich(Editor)
Taylor & Francis (Publisher)
1st Edition
Will be published approx. on 8. November 2007
Book
Paperback/Softback
154 pages
978-0-415-40950-6 (ISBN)
Description
One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful research tool in the functional study of individual genes and their products, and in functional genomics. In RNAi, specific small double-stranded RNA molecules (small interfering RNAs or siRNAs) are introduced into cells to selectively silence certain genes.
RNAi covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent. A key feature of RNAi is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations.
RNAi covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent. A key feature of RNAi is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations.
More details
Series
Language
English
Place of publication
London
United Kingdom
Target group
College/higher education
Dimensions
Height: 246 mm
Width: 174 mm
Weight
286 gr
ISBN-13
978-0-415-40950-6 (9780415409506)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Person
Martin Latterich
Content
1. Methods in RNA Interference 2. RNAi Reagent Design 3. RNA Interference - A Chemical Perspective 4. Validation of RNAi 5. RNA Interference Libraries in Dissecting Molecular Pathways of the Human Cell 6. High-Throughput RNAi in Caenorhabditis elegans - From Molecular Phenotypes to Pathway Analysis 7. RNAi in Xenopus laevis 8. Generation of Transgenic and Knockdown Mice with Lentiviral Vectors and RNAi Techniques 9. RNAi in Fungi


