Fluorescent Molecular Labels and Probes
1st Edition
Will be published approx. 2030
Book
Hardback
500 pages
978-3-527-32720-1 (ISBN)
Description
For newcomers and active researchers in academia and industry, this book provides a structured overview of available probes, while examining and comparing their properties and their performance in the context of specific applications, as well as critically analyzing their validity and identifying important challenges in probe development. As a result, this handbook and ready reference helps researchers to find the best available solution for their experimental problem in terms of fluorophore selection, while also helping to prevent complications and avoid common pitfalls in probe application.
More details
Language
English
Place of publication
Berlin
Germany
Publishing group
Wiley-VCH Verlag GmbH
Target group
Professional and scholarly
Illustrations
60 farbige Abbildungen, 50 s/w Abbildungen
Dimensions
Height: 244 mm
Width: 170 mm
ISBN-13
978-3-527-32720-1 (9783527327201)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Roland Kramer is a Chair of Inorganic Chemistry at University of Heidelberg, Germany. He obtained his PhD from University of Munich in 1994. After a postdoctoral stay in Strasbourg, France, he moved to University of Munster where he was awarded his Habilitation degree in 1997. He was appointed to his current position in Heidelberg in 1999. Research in his group focuses on bioinorganic and bioconjugate chemistry, and more recently on the development of new molecular probes. He coordinates the graduate program "Molecular Probes" at University of Heidelberg. Claudia Kramer is an Organic Chemist.
Content
Major classes of molecular fluorophores (Organic dyes; Transition metal complexes; Lanthanide complexes; Quantum dots and fluorescent polymers will be mentioned briefly) Design and synthesis of labels and probes Important chemical and photophysical properties Signalling mechanisms (Microenvironmental effects; Quenching; Resonance energy transfer; Photoinduced electron transfer; Modulation of fluorescence lifetime; Ratiometric probes; Signal amplification) Sensitivity and detection limits Target selectivity and molecular recognition Major fields of application (Biomolecule detection, Medical diagnostics, Enzyme assays, High-throughput screening, Cellular imaging and microscopy) Fluorescent labels (Proteins, Antibodies; Proteins in live cells; Nucleic acids; Lipids; Application to cellular imaging; Application to molecular imaging) Fluorescent Probes (pH; Metal ions; Simple anions; Metabolites; Reactive Oxygen Species (including NO); Enzyme substrates; Proteins in vitro; Proteins in live cells; Nucleic acids; Cellular substructures) Labels and Probes for ultrahigh sensitivity and resolution (Multiple-fluorophore labels, Fluorescence ELISA, Tyramide signal amplification, Enyzme labeled fluorescence, Probes for ultra-high resolution optical microscopy, Probes for single molecule fluorescence spectroscopy) Challenges in probe development