
Introduction to Proteomics
Introduction to Methods and Applications
Wiley (Publisher)
Published on 7. November 2008
Book
Hardback
352 pages
978-0-470-05535-9 (ISBN)
Description
Everything you need to begin conducting your own proteomics research
Proteomics: Introduction to Methods and Applications is the ideal introduction to the field of proteomics, giving readers the skills and knowledge needed to study proteins, their structure, and their function in cells, tissues, and organisms. Written by experienced researchers, the text not only covers all the basic concepts, techniques, and applications, it also offers readers insights and practical tips based on the authors' many years of hands-on work in proteomics research. Moreover, the text has been carefully edited to ensure a thorough, consistent, and highly readable approach throughout all the chapters.
This text offers everything readers need to begin conducting their own proteomics research, including:
* A full chapter dedicated to laboratory exercises and protocols to help readers apply their skills
* Step-by-step explanations of key analytical methods, including mass spectrometry, sample preparation, chromatographic and electrophoretic separation, and micro-total analysis systems
* Complete coverage of bioinformatics, including an introduction to important proteomics databases
* A glossary defining the key terms in the field
* An appendix with an annotated list of Internet resources, information on post-translational modifications, and the masses and compositions of amino acid residues
This text is designed for upper-level undergraduate and graduate courses in proteomics. Upon successful completion of the text, students are able to apply their skills and knowledge to solve problems in such fields as biotechnology, drug discovery, and medical research.
Reviews / Votes
"This work will be a useful tool for students of biotechnology, and it will also serve as an excellent reference for researchers interested in applying proteomics to their own investigations." (CHOICE, August 2009)More details
Product info
gebunden
Series
Edition
1. Auflage
Language
English
Place of publication
United States
Publishing group
John Wiley & Sons Inc
Target group
College/higher education
Product notice
sewn/stitched
Cloth over boards
Dimensions
Height: 241 mm
Width: 158 mm
Thickness: 24 mm
Weight
684 gr
ISBN-13
978-0-470-05535-9 (9780470055359)
Schweitzer Classification
Persons
Agnieszka Kraj, PHD, is an Associate Professor of the Neurobiochemistry Department in the Faculty of Chemistry at Jagiellonian University, Poland.
Jerzy Silberring, PHD, is the Head of the Neurobiochemistry Department in the Faculty of Chemistry as well as the former deputy head of the Regional Laboratory for Physicochemical Analyses at Jagiellonian University, Poland. Dr. Silberring is coeditor of Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research and Mass Spectrometry: Instrumentation, Interpretation, and Applications (both from Wiley).
Content
Foreword.
1. Introduction to Proteomics and Strategy for Proteins Identification.
2. Sample Preparation.
3. Chromatographic Separation Methods.
3.1. Liquid Chromatography Linked to Mass Spectrometry.
3.2. Multidimensional Liquid Chromatography Combined with Mass Spectrometry.
4. Electrophoretic Separation Methods.
4.1. One-Dimensional Gel Electrophoresis.
4.2. Two-Dimensional Gel Electrophoresis.
5. Capillary Electrophoresis.
6. Protein Blotting.
7. Introduction to Proteins and Peptides Mass Spectrometry.
7.1. Electrospray Ionization (ESI).
7.2. Matrix-Assisted Laser Desorption Ionization (MALDI).
7.3. Imaging Mass Spectrometry.
7.4. Fragmentation and Sequencing in Proteomics.
7.5. Protein Identification by Mass Spectrometry.
7.6. Chemical Derivatization for Sequence Analysis.
8. Protein Modifications.
9. Microfluidics.
10. Proteomics.
10.1. Quantitative Proteomics.
10.2. Structural Proteomics.
10.3. Functional Proteomics.
10.4. Clinical Proteomics.
11. Bioinformatics.
12. Appendix.
13. The Laboratory Practicals.
13.1. Polyacrylamide Gel Electrophoresis under Denaturing Conditions.
13.2. Preparation of the In-Gel Separated Proteins for Mass Spectrometry.
13.3. Bioinformatic Tools for Protein Identification.
13.4. LC-MS and LC-MS/MS - Proteomics Applications.
13.5. Enzyme Kinetics.
13.6. Internet Databases.