Advanced Techniques in Biological Electron Microscopy III
Published on 1. May 1986
Book
Paperback/Softback
XIV, 278 pages
978-3-540-16400-5 (ISBN)
Description
This volume is a continuation of two prior books on advanced electron microscope techniques. The purpose of this series has been to provide in depth analyses of methods which are considered to be at the leading edge of electron microscopic research procedures with applications in the biological sciences. The mission of the present volume remains that of a source book for the research practitioner or advanced student, especially one already well versed in basic electron optical methods. It is not meant to provide in troductory material, nor can this modest volume hope to cover the entire spectrum of advanced technology now available in electron microscopy. In the past decade, computers have found their way into many research laboratories thanks to the enormous increase in computing power and stor age available at a modest cost. The ultrastructural area has also benefited from this expansion in a number of ways which will be illustrated in this volume. Half of the contributions discuss technologies that either directly or indirectly make extensive use of computer methods.
More details
Edition
Softcover reprint of the original 1st ed. 1986
Language
English
Place of publication
Berlin
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Research
Illustrations
65 s/w Abbildungen
XIV, 278 p. 65 illus.
Dimensions
Height: 235 mm
Width: 155 mm
Thickness: 17 mm
Weight
452 gr
ISBN-13
978-3-540-16400-5 (9783540164005)
DOI
10.1007/978-3-642-71135-0
Schweitzer Classification
Other editions
Additional editions
E-Book
12/2012
Springer
€96.29
Available for download
Persons
Content
Three-Dimensional Reconstruction of Nonperiodic Macromolecular Assemblies from Electron Micrographs.- 1 Introduction.- 2 Methods for Obtaining Projection Data.- 3 Methods of Three-Dimensional Reconstruction.- 4 Experimental Results.- 5 Conclusions.- References.- High Resolution Biological X-Ray Microanalysis of Diffusable Ions.- 1 Introduction.- 2 Principles of the Technique.- 3 Specimen Preparation.- 4 Quantitation.- 5 Applications in the Analysis of Diffusable Ions.- 6 Conclusion.- References.- Metal Deposition by High-Energy Sputtering for High Magnification Electron Microscopy.- 1 Introduction.- 2 Methods.- 3 Metal Deposition.- 4 Conclusion.- References.- Computer Programs for Biological Stereology.- 1 Introduction.- 2 Point Counting Programs.- 3 Digitizing Programs.- 4 Special Purpose Stereology Programs.- 5 Concluding Comments.- References.- A Guide to Fracture Label: Cytochemical Labeling of Freeze-Fractured Cells.- 1 Introduction.- 2 Experimental Procedures.- 3 Electron Microscopy.- 4 Interpretation.- References.- The Preparation of Colloidal Gold Probes and Their Use as Marker in Electron Microscopy.- 1 Introduction.- 2 The Preparation and Storage of Colloidal Gold Sols.- 3 The Production, Purification, and Storage of Gold Probes.- 4 Quality Control and Analysis of Gold Sols and Probes.- 5 Critical Evaluation of the Use of Gold Probes in Selected Marking Techniques.- 6 Conclusions.- References.