Advanced Microscopy
Photo-Thermal and Induced-Raman Microscopy
1st Edition
Published on 15. October 2024
Book
Hardback
434 pages
978-1-032-75885-5 (ISBN)
Description
This book covers the principle, structure, enhancement of sensitivity and resolution power of photothermal and Raman microscopies. It includes real-world applications to biological and medical targets.
Advanced Microscopy: Photo-Thermal and Induced-Raman Microscopy introduces clear descriptions of various Raman processes such as spontaneous, stimulates, coherent anti-Stokes Raman (CARS), Raman loss and Stokes Raman (gain). It covers pump-probe microscopies using actinic (pump) laser and sensing (probe) laser resulting in improvement due to intrinsic nonlinearity, which provides an advantage in the imaging of nonfluorescent targets. The author also provides solutions to noise and sensitivity problems which are two of the most important concerns in the microscopy applications. Finally, the book also draws direct comparisons of the advantages and drawbacks of a Raman microscopes in comparison with photothermal microscopes.
The book will be useful to researchers and non-specialists in biomedical fields using optics and electronics relevant to (optical) microscopes. It will also be a helpful resource to graduate students in the fields of biology and medical research who are using photothermal microscopes in their research.
Advanced Microscopy: Photo-Thermal and Induced-Raman Microscopy introduces clear descriptions of various Raman processes such as spontaneous, stimulates, coherent anti-Stokes Raman (CARS), Raman loss and Stokes Raman (gain). It covers pump-probe microscopies using actinic (pump) laser and sensing (probe) laser resulting in improvement due to intrinsic nonlinearity, which provides an advantage in the imaging of nonfluorescent targets. The author also provides solutions to noise and sensitivity problems which are two of the most important concerns in the microscopy applications. Finally, the book also draws direct comparisons of the advantages and drawbacks of a Raman microscopes in comparison with photothermal microscopes.
The book will be useful to researchers and non-specialists in biomedical fields using optics and electronics relevant to (optical) microscopes. It will also be a helpful resource to graduate students in the fields of biology and medical research who are using photothermal microscopes in their research.
More details
Language
English
Place of publication
London
United Kingdom
Publishing group
Taylor & Francis Ltd
Target group
College/higher education
Professional and scholarly
Postgraduate and Professional Reference
Illustrations
28 s/w Tabellen, 10 Farbfotos bzw. farbige Rasterbilder, 73 s/w Zeichnungen, 10 farbige Zeichnungen, 66 s/w Photographien bzw. Rasterbilder, 20 farbige Abbildungen, 139 s/w Abbildungen
28 Tables, black and white; 10 Line drawings, color; 73 Line drawings, black and white; 10 Halftones, color; 66 Halftones, black and white; 20 Illustrations, color; 139 Illustrations, black and white
Dimensions
Height: 240 mm
Width: 161 mm
Thickness: 28 mm
Weight
825 gr
ISBN-13
978-1-032-75885-5 (9781032758855)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Book
approx. 07/2026
1st Edition
CRC Press
€66.00
Not yet published
E-Book
10/2024
1st Edition
CRC Press
€73.99
Available for download
E-Book
10/2024
1st Edition
CRC Press
€73.99
Available for download
Person
Takayoshi Kobayashi was born in Niigata Prefecture in 1944. He is a professor emeritus at the University of Tokyo, from which he previously graduated with Bachelor's, Master's, and Doctorate degrees. He joined the Institute of Physical and Chemical Research (Riken), and between 1977 and 1979, he was a temporary member of the Technical Staff at Bell Laboratories. In 1980 he joined the Department of Physics at the University of Tokyo as an associate professor and was promoted to full professor in 1994. In March 2006 he retired from the university and moved to the Department of Applied Physics and Chemistry at the University of Electro-Communications, in Tokyo. He was appointed Chair Professor and the Director of the National Chiao-tung University in 2006, which became the National Yang-Ming Chiao-Tung University in 2021. His research interests include quantum electronics, laser physics, femtosecond spectroscopy, ultrafast nonlinear optics, quantum optics, chemical physics, quantum information science and technology.
Content
Chapter 1 Introduction
Chapter 2.1.1 Optimal Detection Angle in Sub-diffraction Resolution Photothermal Microscopy: Application for High-sensitivity Imaging of Biological Tissues
Chapter 2.1.2 Resolution Enhanced Pump-probe Microscope with a Spatial Filter
Chapter 2.1. Reduction of Distortion in Photothermal Microscopy and its Application to the High-resolution Three-dimensional Imaging of Nonfluorescent Tissues
Chapter 2.2.1 Numerical Study of the Subtraction Threshold for Fluorescence Difference Microscopy
Chapter 2.2.2 Sub-Diffraction-Limit Imaging Using Mode Multiplexing
Chapter 2.2.3 Polarization Modulation for Fluorescence Emission Difference Microscopy
Chapter 2.2.4 Numerical Study of Superresolved Optical Microscopy with Partly Staggered Beams
Chapter 2.2.5 Resolution Enhancement of Pump-probe Microscopy with an Inverse-annular Spatial Filter
Chapter 3.1 Sub-Diffraction Resolution Pump-probe Microscopy with Shot-Noise Limited Sensitivity Using Laser Diodes
Chapter 3.2 Sensitivity Enhancement of Photothermal Microscopy with Radially Segmented Balanced Detection
Chapter 3.3 Fast 3D Visualization of Endogenous Brain Signals with High-sensitivity Laser Scanning Photothermal Microscopy
Chapter 5.2 Label-Free Imaging of Melanoma with Nonlinear Photothermal Microscopy3
Chapter 5.3 Noninvasive, Label-free, Three-dimensional Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiate Malignant Melanoma from Benign Tumor Tissue
Chapter 5.4 Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue
Chapter 6.1 Development of a Multiplex Stimulated Raman Microscope for Spectral Imaging through Multi-channel Lock-in Detection
Chapter 6.2 Multiplex Stimulated Raman Imaging with White Probe-light from a Photonic-crystal Fiber and with Multi-wavelength Balanced Detection
Chapter 6.3 Theoretical Description for Nonlinear Dynamic Light Scattering Based on Stimulated Raman Effect (NLDLS-SRS)
Chapter 2.1.1 Optimal Detection Angle in Sub-diffraction Resolution Photothermal Microscopy: Application for High-sensitivity Imaging of Biological Tissues
Chapter 2.1.2 Resolution Enhanced Pump-probe Microscope with a Spatial Filter
Chapter 2.1. Reduction of Distortion in Photothermal Microscopy and its Application to the High-resolution Three-dimensional Imaging of Nonfluorescent Tissues
Chapter 2.2.1 Numerical Study of the Subtraction Threshold for Fluorescence Difference Microscopy
Chapter 2.2.2 Sub-Diffraction-Limit Imaging Using Mode Multiplexing
Chapter 2.2.3 Polarization Modulation for Fluorescence Emission Difference Microscopy
Chapter 2.2.4 Numerical Study of Superresolved Optical Microscopy with Partly Staggered Beams
Chapter 2.2.5 Resolution Enhancement of Pump-probe Microscopy with an Inverse-annular Spatial Filter
Chapter 3.1 Sub-Diffraction Resolution Pump-probe Microscopy with Shot-Noise Limited Sensitivity Using Laser Diodes
Chapter 3.2 Sensitivity Enhancement of Photothermal Microscopy with Radially Segmented Balanced Detection
Chapter 3.3 Fast 3D Visualization of Endogenous Brain Signals with High-sensitivity Laser Scanning Photothermal Microscopy
Chapter 5.2 Label-Free Imaging of Melanoma with Nonlinear Photothermal Microscopy3
Chapter 5.3 Noninvasive, Label-free, Three-dimensional Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiate Malignant Melanoma from Benign Tumor Tissue
Chapter 5.4 Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue
Chapter 6.1 Development of a Multiplex Stimulated Raman Microscope for Spectral Imaging through Multi-channel Lock-in Detection
Chapter 6.2 Multiplex Stimulated Raman Imaging with White Probe-light from a Photonic-crystal Fiber and with Multi-wavelength Balanced Detection
Chapter 6.3 Theoretical Description for Nonlinear Dynamic Light Scattering Based on Stimulated Raman Effect (NLDLS-SRS)