
Lipid-Protein Interactions
Methods and Protocols
Jörg H. Kleinschmidt(Editor)
Humana Press Inc.
2nd Edition
Published on 14. August 2020
Book
Paperback/Softback
XIV, 682 pages
978-1-4939-9514-1 (ISBN)
Description
This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.
Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.
More details
Product info
Book
Series
Edition
2nd ed. 2019
Language
English
Place of publication
NY
United States
Target group
Professional and scholarly
Edition type
Revised edition
Illustrations
92 farbige Abbildungen, 55 s/w Abbildungen
92 Illustrations, color; 55 Illustrations, black and white; XIV, 682 p. 147 illus., 92 illus. in color.
Dimensions
Height: 254 mm
Width: 178 mm
Thickness: 38 mm
Weight
1293 gr
ISBN-13
978-1-4939-9514-1 (9781493995141)
DOI
10.1007/978-1-4939-9512-7
Schweitzer Classification
Other editions
Additional editions

Book
06/2019
2nd Edition
Humana Press Inc.
€246.09
Shipment within 15-20 days
Content
Preface...Table of Contents...Contributing Authors...
1. Multiscale Modeling and Simulation Approaches to Lipid-Protein InteractionsRoland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine, Jan K. Marzinek, Firdaus Samsudin, Jane R. Allison, Syma Khalid, and Peter J. Bond
2. Quartz Crystal Microbalances as Tools for Probing Protein-Membrane InteractionsSøren B. Nielsen and Daniel E. Otzen
3. Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid MembranesAleksandra Sakanovic, Vesna Hodnik, and Gregor Anderluh
4. Thermodynamic Analysis of Protein-Lipid Interactions by Isothermal Titration CalorimetryMusti J. Swamy, Rajeshwer S. Sankhala, and Bhanu Pratap Singh
5. Differential Scanning Calorimetry of Protein-Lipid InteractionsOlga Cañadas and Cristina Casals
6. Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force MicroscopeK. Tanuj Sapra
7. Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel-Electrophoresis Andre Schüßler, Sascha Herwig, and Jörg H. Kleinschmidt
8. Optimized Negative-Staining Protocol for Lipid-Protein Interactions Investigated by Electron MicroscopyJianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie, Jinping Li, and Gang Ren
9. Probing Heterogeneous Lipid Interactions with Membrane Proteins using Mass SpectrometryJohn W. Patrick and Arthur Laganowsky
10. Protein Microarrays and Liposome: A Method for Studying Lipid-Protein InteractionsSamuel Herianto, Chien-Sheng Chen, and Heng Zhu
11. Structural Investigations of Protein-Lipid Complexes using Neutron ScatteringLuke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, and Jeremy H. Lakey
12. Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid EnvironmentKoichi Matsuo and Kunihiko Gekko
13. FTIR Analysis of Proteins and Protein-Membrane InteractionsSuren A. Tatulian
14. UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and DynamicsDeeAnn K. Asamoto and Judy E. Kim
15. Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence QuenchingGregory A. Caputo and Erwin London
16. Förster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid SelectivityLuís M. S. Loura, Manuel Prieto, and Fábio Fernandes
17. A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid BilayersEvan L. Taylor, Kumud Raj Poudel, and James A. Brozik
18. Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in MembranesViktoria Betaneli, Jonas Mücksch, and Petra Schwille
19. Membrane Pore Formation by Peptides Studied by Fluorescence TechniquesSuren A. Tatulian and Nabin Kandel
20. Folding and Insertion of ß-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence SpectroscopyLisa Gerlach, Omkolsum Gholami, Nicole Schürmann, and Jörg H. Kleinschmidt
21. EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid BilayersEnrica Bordignon, Svetlana Kucher, and Yevhen Polyhach
22. Studying Lipid-Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled LipidsTibor Pa´li and Zolta´n Ko´ta
23. Solid-State NMR Approaches to Study Protein Structure and Protein-Lipid InteractionsChristopher Aisenbrey, Evgeniy S. Salnikov, Jesus Raya, Matthias Michalek, and Burkhard Bechinger
24. Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in Lipid EnvironmentAshish Arora
25. Nanodiscs as a New Tool to Examine Lipid-Protein InteractionsIlia G. Denisov, Mary A. Schuler, and Stephen G. Sligar
1. Multiscale Modeling and Simulation Approaches to Lipid-Protein InteractionsRoland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine, Jan K. Marzinek, Firdaus Samsudin, Jane R. Allison, Syma Khalid, and Peter J. Bond
2. Quartz Crystal Microbalances as Tools for Probing Protein-Membrane InteractionsSøren B. Nielsen and Daniel E. Otzen
3. Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid MembranesAleksandra Sakanovic, Vesna Hodnik, and Gregor Anderluh
4. Thermodynamic Analysis of Protein-Lipid Interactions by Isothermal Titration CalorimetryMusti J. Swamy, Rajeshwer S. Sankhala, and Bhanu Pratap Singh
5. Differential Scanning Calorimetry of Protein-Lipid InteractionsOlga Cañadas and Cristina Casals
6. Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force MicroscopeK. Tanuj Sapra
7. Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel-Electrophoresis Andre Schüßler, Sascha Herwig, and Jörg H. Kleinschmidt
8. Optimized Negative-Staining Protocol for Lipid-Protein Interactions Investigated by Electron MicroscopyJianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie, Jinping Li, and Gang Ren
9. Probing Heterogeneous Lipid Interactions with Membrane Proteins using Mass SpectrometryJohn W. Patrick and Arthur Laganowsky
10. Protein Microarrays and Liposome: A Method for Studying Lipid-Protein InteractionsSamuel Herianto, Chien-Sheng Chen, and Heng Zhu
11. Structural Investigations of Protein-Lipid Complexes using Neutron ScatteringLuke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, and Jeremy H. Lakey
12. Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid EnvironmentKoichi Matsuo and Kunihiko Gekko
13. FTIR Analysis of Proteins and Protein-Membrane InteractionsSuren A. Tatulian
14. UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and DynamicsDeeAnn K. Asamoto and Judy E. Kim
15. Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence QuenchingGregory A. Caputo and Erwin London
16. Förster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid SelectivityLuís M. S. Loura, Manuel Prieto, and Fábio Fernandes
17. A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid BilayersEvan L. Taylor, Kumud Raj Poudel, and James A. Brozik
18. Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in MembranesViktoria Betaneli, Jonas Mücksch, and Petra Schwille
19. Membrane Pore Formation by Peptides Studied by Fluorescence TechniquesSuren A. Tatulian and Nabin Kandel
20. Folding and Insertion of ß-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence SpectroscopyLisa Gerlach, Omkolsum Gholami, Nicole Schürmann, and Jörg H. Kleinschmidt
21. EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid BilayersEnrica Bordignon, Svetlana Kucher, and Yevhen Polyhach
22. Studying Lipid-Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled LipidsTibor Pa´li and Zolta´n Ko´ta
23. Solid-State NMR Approaches to Study Protein Structure and Protein-Lipid InteractionsChristopher Aisenbrey, Evgeniy S. Salnikov, Jesus Raya, Matthias Michalek, and Burkhard Bechinger
24. Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in Lipid EnvironmentAshish Arora
25. Nanodiscs as a New Tool to Examine Lipid-Protein InteractionsIlia G. Denisov, Mary A. Schuler, and Stephen G. Sligar