RT-PCR Protocols

Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples Susanne Greber-Platzer, Brigitte Balcz, Christine Fleischmann, and Gert Lubec Detection of mRNA Expression and Alternative Splicing in a Single Cell Tsutomu Kumazaki Nested RT-PCR: Sensitivity Controls are Essential to Determine the Biological Significance of Detected mRNA Triona Goode, Wen-Zhe Ho, Terry O'Connor, Sandra Busteed, Steven D. Douglas, Fergus Shanahan, and Joe O'Connell Part III. Quantitative RT-PCR Quantitative RT-PCR: A Review of Current Methodologies Caroline Joyce Rapid Development of a Quantitative-Competitive (QC) RT-PCR Assay Using a Composite Primer Approach Joe O'Connell, Aileen Houston, Raymond Kelly, Darren O'Brien, Aideen Ryan, Michael W. Bennett, and Kenneth Nally Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products Franz Bachmair, Christian G. Huber, and Guenter Daxenbichler Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR Kaisa Nieminen, Markus Halminen, Matti Waris, Mika Mäkelä, Johannes Savolainen, Minna Sjöroos, and Jorma Ilonen Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytes Jian-Ping Lai, Steven D. Douglas, and Wen-Zhe Ho Part IV. Detection and Analysis of RNA Viruses Detection and Quantification of the Hepatitis C Viral Genome Liam J. Fanning Semi-Quantitative Detection of Hepatitis C Virus RNA by 'Real-Time' RT-PCR Joerg F. Schlaak RT-PCRfor the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus Brian Mullan, Liam Fanning, Fergus Shanahan, and Daniel G. Sullivan Part V. In Situ Localization of mRNA Expression In Situ Immuno-PCR: A Newly Developed Method for Highly Sensitive Antigen Detection In Situ Yi Cao RT-PCR from Laser Capture Microdissected Samples Tatjana Crnogorac-Jurcevic, Torsten O. Nielsen, and Nick R. Lemoine Mycobacterium Paratuberculosis Detected by Nested PCR in Intestinal Granulomas Isolated by Laser Capture Microdissection in Cases of Crohn's Disease Paul Ryan, Simon Aarons, Michael W. Bennett, Gary Lee, Gerald C. O'Sullivan, Joe O'Connell, and Fergus Shanahan RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization Joe O'Connell Part VI. Differential mRNA Expression Amplified RNA for Gene Array Hybridizations Valentina I. Shustova and Stephen J. Meltzer Semiquantitative Determination of Differential Gene Expression in Primary Tumors and Matched Metastases by RT-PCR: Comparison with Other Methods Benno Mann and Christoph Hanski Part VII. Genetic Analysis Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay Frank Waldron-Lynch, Claire Adams, Michael G. Molloy, and Fergal O'Gara Part VIII. RT-PCR in Immunology Detection of Clonally Expanded T Cells by RT-PCR-SSCP and Nucleotide Sequencing of T Cell Receptor b-CDR3 Regions Manae Suzuki Kurokawa, Kusuki Nishioka, and Tomohiro Kato Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B Cells Nadège Gruel, Beatrix Kotlan, Marie Beuzard, and Jean-Luc Teillaud Generation of Murine scFv Intrabodies from B Cell Hybridomas Chang Hoon Nam, Sandrine Moutel, and Jean-Luc Teillaud Quantitation of mRNA Levels by