The Baculovirus Expression System
A Laboratory Guide
Published in April 1992
Book
Hardback
248 pages
978-0-412-37150-9 (ISBN)
Description
As a result of recent major advances in molecular biological techniques, the use of baculovirus expression systems is now widespread and publications reviewing the techniques are numerous. The aim of this practical manual is to give first-time users the relevant background information and detailed laboratory methods. Methods and protocols are provided and more detailed applications are highlighted for the benefit of the more experienced user. Emphasis is placed on practical applications, interpretation of results and potential problems and pitfalls.
More details
Language
English
Place of publication
London
United Kingdom
Target group
College/higher education
Professional and scholarly
Product notice
sewn/stitched
Cloth over boards
Dimensions
Height: 241 mm
Width: 160 mm
Thickness: 20 mm
Weight
541 gr
ISBN-13
978-0-412-37150-9 (9780412371509)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
Book
07/1997
2nd Edition
Chapman and Hall
€68.09
Article is exhausted; no reprint
Additional editions
Book
11/2012
Springer
€80.24
Shipment within 15-20 days
Content
The baculoviruses - introduction; the development of baculovirus expression vectors; processing of foreign proteins synthesized using baculovirus vectors in insect cells; construction of transfer vectors containing the foreign gene; insect cell culture media and maintenance of insect cell lines; propagation, titration and purification of AcMNPV in cell culture; production and selelction of recombinant virus; characterization of recombinant viruses; scaling up the production of recombinant proteins in insect cells - laboratory bench level; propagation of baculoviruses in insect larvae.