Advances in Membrane Biochemistry and Bioenergetics

Tsoo E. King.- My Teachers and I.- Reconstitution as a Research Programme: T. E. King 1956-1986.- Plenary Session.- Respiratory Chain Systems in Theory and Practice.- 1. Mitochondrial Electron Transfer.- Introductory Remarks.- Flavoproteins: Correlations of Structure and Function.- Mitochondrial Electron Transport: The Random Collision Model.- Lateral Diffusion as a Rate-Limiting Step in Mitochondrial.Electron Transport.- The Pool Function of Ubiquinone in the Mitochondrial Respiratory Chain: Role of Lateral Diffusion.- Effects of Long Chain Alkyl Compounds on Submitochondrial Particles: Mechanistic Differences between NADH and Succinate Oxidases.- Purification and Properties of the Rotenone-Insensitive NADHiQ6 Oxidoreductase from Saccharomyces cerevisiae.- An Ubiquinone-Binding Protein, A Constituent of Iron-Sulfur Fragment Isolated from Mitochondrial NADH-Ubiquinone Reductase (Complex I).- Construction of Yeast Mutants of QH2 Cytochrome c Oxidoreductase: Requirements of the 11, 14 and 17 kDa Subunitsf but Not the 40 kDa Subunitf for Enzymatic Activity.- Electron Transfer and Spectral Changes in Cytochrome b Studied in Mitochondria from Coenzyme Q-Deficient Mutants of the YeastSaccharomycescerevisiaeReconstituted with Coenzyme Q Analogues.- Structural and Functional Features of the Eleven Constituent Proteins of the Mammalian Ubiquinol: Cytochrome c Reductase.- A Mitochondrial Protein Essential for Interaction between Cardiac Cytochromes c1 and c: The Hinge Protein.- A Function of the Hinge Protein c1-c.- The Thiopropionyl Derivatives of Cytochrome c.- Monoclonal Antibodies of Mammalian Cytochrome Oxidase and its Protein Subunits.- Biochemical Characterization of Boar Sperm Cytochrome Oxidase.- Molecular Events in Cytochrome c Oxidase.- The Cyanide-Resistant Oxidase in Higher Plant Mitochondria.- 2. Bacterial-Chloroplast Electron Transfer.- Comparative Studies on the NADH-Q Oxidoreductase Segment of the Bacterial Respiratory Chain.- T. OshimaPathways of Electron Transfer in Desulfovibrio.- Studies on A Novel Flavodoxin from the Repiratory Mutant TZN-200 from Azotobacter vinelandii.- Electron Transfer Components of Mangansese Oxidizing Bacteria.- Biochemical and Genetic Approaches to Elucidating the Mechanism of Respiration and Energy Transduction inParacoccus denitrificans.- Reconstitution of Function in Fatty-Acid Inactivated Photosystem 2.- Electron Transport in the Cytochrome b6f Complex.- 3. Energy Coupling And Ion Transport.- pH-Dependence of the Maximal Velocity of Cytochrome c Oxidase: Relation to Proton and Respiratory Control.- Control of Proteoliposomal Cytochrome Oxidase: Normal and Inverted Orientations.- Cytochrome c Peroxidase Activity of Cytochrome Oxidase and its Coupling to Proton Pumping.- Characteristics of the Protonmotive Activity of the Cytochrome Chain of Mitochondria.- Dependence of the 3-OH-Butyrate Dehydrogenase and Cytochrome c Oxidase Reactions- on Intramitochondrial pH.- Role of Coupling Factor B in the FO Proton Channel.- ATP Synthesis and Ion Gradients in the Methanogenic Bacteria.- Energy Transfer in Solute Carrier Transport.- Interaction of Quinine with Mitochondrial K+ Transport Mechanisms.- Outer Membrane Lysis Increases Accessibility of Cationic Drugs to the Inner Mitochondial Membrane.- 4. Biophysical Techrliques.- Optical and X-Ray Techniques in the Study of Rapid Ligand Binding: A Ligand "Docking" Site in the Reaction of Mb and CO at 40 K.- X-Ray Absorption Studies of Oriented Cytochrome Oxidase.- Newly Developed Time Resolved EPR Techniques for the Study of Cytochrome c Oxidaie.- EPR and Electron Spin Echo Studies of Iron-Sulfur Clusters S-I and S-2 in Bovine Heart Succinate Dehydrogenase.- New Avenues in Electron Microscopy of Transmembranous Biomolecular Assemblies.- Structural Influences on the Electrochemisty of Ubiquinone.- 5. Related Enzyme Systems.- Enzymatic Synthesis of Acetono-CoA: A Competitive Inhibitor of Acetyl-CoA with Citrate Synthase.- Purification and Properties of Two Isoenzymes of Tench Liver Arginase.- Enzymes at Oil-Water Interfaces and in Organic Solvents.- Conformational Changes at the Active Site of D-Glyceraldehyde-3-Phosphate Dehydrogenase during Unfolding in Guanidine Solution.- Acute Stimulation by Peptide Hormones of Mitochondrial Steroid Hormone Synthesis: Production of A Similar Protein in Three Steroidogenic Tissues.- Interaction of Steroid Substrates with Cytochrome P-450C21-Proteoliposomes.- Regulation of Cholestrol 7 ?-Hydroxylase.- 6. Clinical Aspects.- An Overview of Biochemical and Clinical Research on Coenzyme Q with Emphasis on Cardiomyopathy and Muscle Dystrophy.- Membrane Damage Provoked by Lipid Peroxides.- Oxidation-Reduction Associated with Inhaled NO2 Absorption.- The Reconstitution of Biologically Active Glycoproteins into Large Liposomes: Use as a Delivery Vehicle to Animal Cells.- Symposium Participants.