Nonradioactive Analysis of Biomolecules

1 General Aspects of Nonradioactive Analysis of Biomolecules.- 2 Overview of Nonradioactive Labeling Systems.- 3 Overview on the Digoxigenin:Anti-Digoxigenin (DIG) System.- 4 Labeling and Detection of Nucleic Acids.- 5 Labeling and Detection of Proteins and Glycoproteins.- 6 Labeling and Detection of Nucleic Acids.- 7 Labeling of Proteins DuringIn VitroTranslation.- 8 Labeling and Detection of Proteins and Glycoproteins.- 9 In Vitro Labeling of DNA Probes with 5-BrdU.- 10 AAF-labeling of DNA and Oligonucleotides.- 11 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Conjugates.- 12 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection.- 13 The SNAP System.- 14 Overview on Nonradioactive Detections Systems.- 15 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems.- 16 Indigo / Tetrazolium Dyes.- 17 Azo Dyes.- 18 Chemiluminescent Detection with Horseradish Peroxidase and Luminol.- 19 Chemiluminescence: Properties of 1,2-Dioxetane Chemiluminescence.- 20 Electrochemiluminescence: Ruthenium Complexes.- 21 Bioluminescence: D-Luciferin as Substrate.- 22 Labeling of Biomolecules with Fluorophores.- 23 Time-Resolved Fluorescence.- 24 Overview of Amplification Systems.- 25 Amplification of Nucleic Acids by Polymerase Chain Reaction: Overview on Basic Principles.- 26 PCR: Overview on Application Formats in Research and Clinical Diagnosis.- 27 PCR Amplification for the Generation of DIG-Labeled Probes.- 28 In Situ PCR Amplification of cDNA.- 29 SDA Target Amplification.- 30 In Situ Strand Displacement Amplification.- 31 Isothermal Amplification of RNA by Transcription-Mediated Amplification (TMA).- 32 Ligase Chain Reaction.- 33 Branched DNA (bDNA) Technology.- 34 rDNA Amplification: Application of 16S rDNA-Based Methods for Bacterial Identification.- 35 PRINS: Primed In Situ Labeling and Hybridization in one Step.- 36 Overview on Amplification Formats.- 37 Overview on Factors Influencing Nucleic Acid Hybridization.- 38 Dot, Southern, and Northern Blots.- 39 PNA Pre-Gel Hybridization - An Alternative to Southern Blotting.- 40 Multilocus DNA Fingerprinting Using Nonradioactively Labeled Oligonucleotide Probes Specific for Simple Repeat Elements.- 41 Detection of Proteins and Glycoproteins on Western Blots.- 42 PNA as Specific Probe for In Situ Hybridization to Metaphase Chromosomes.- 43 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays.- 44 Non-Fluorescent Differentiation of Viral and Chromosomal Nucleic Acids in Individual Nuclei.- 45 Virus Detection in Biopsy Specimens.- 46 Mapping of Polytene Chromosomes.- 47 Fluorescence In Situ Hybridization on Banded Chromosomes.- 48 Spectral Karyotyping: New Tools for Multicolor Chromosome Analysis.- 49 Chromosome Analysis by Multiplex-FISH (M-FISH).- 50 Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos.- 51 Double Labeling of mRNA and Proteins in Drosophila Embryos.- 52 Detection of DNA/RNA Target/Probe Complexes with DNA/RNA-Specific Antibodies.- 53 Molecular Beacons: Hybridization Probes for Detection of Nucleic Acids in Homogeneous Solutions.- 54 Detection of DIG-Labeled Amplicons on Streptavidin-Coated Microtiter Plates.- 55 A Fluorogenic PCR-Based Assay for the Rapid Detection of Salmonella.- 56 PCR-Coupled Activation of Intercalating Dyes.- 57 Simultaneous SDA and Fluorescence Polarization Detection.- 58 Large-Scale Genomic Sequencing Using Four Color Fluorescent Detection - Principles and Protocols.- 59 DIG DNA Sequencing with Chemiluminescent or Dye Substrates.- 60 DNA Sequencing: Chemiluminescent Detection with the 1,2 Dioxane CSPD.- 61 Direct-Blotting-Electrophoresis (DBE) for DNA Sequencing.- 62 Sequence Analysis of Nucleic Acids by Mass Spectrometry.- 63 Mismatch Analysis by PNA Arrays.- 64 Oligonucleotide Arrays for the Detection of ras Mutations.- 65 Use of Oligonucleotide Arrays for Gene Expression Monitoring.