The Use of High Performance Liquid Chromatography in Receptor Biochemistry
Published on 10. November 1989
Book
Hardback
266 pages
978-0-471-56231-3 (ISBN)
Description
This text offers a concise background in HPLC methodology and includes illustrative examples of some of the key advances made in the receptor field through application of this chromatographic technique. A brief historical overview puts into perspective the specific state-of-the-art of HPLC laboratory applications that are contained in the book's chapters. Technical contributions discuss how best to select the HPLC mode for a particular biochemical problem, including the most effective use of a particularly sensitive technique, microbore HPLC for synthesis, purification and characterization of synthetic peptides. The text will benefit biochemists and molecular biologists.
More details
Series
Language
English
Place of publication
New York
United States
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Illustrations
Ill.
Dimensions
Height: 261 mm
Width: 184 mm
Weight
765 gr
ISBN-13
978-0-471-56231-3 (9780471562313)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
The isolation of endogenous peptides, with an emphasis on their biologic activity; the application of HPLC to receptor mapping (illustrated with case studies of the structural features of receptors discovered with this technique); the use of immunoaffinity chromatography to recover biologically active proteins; the application of a wide range of HPLC modes to the study of steroid hormone receptors; a highly detailed strategy for isolating small amounts of proteins and peptides for microsequence analysis.