The world of RNA
Transcripts, Transcriptomes, Transcriptomics
Wiley-VCH (Publisher)
1st Edition
Will be published approx. 2030
Book
Hardback
600 pages
978-3-527-33193-2 (ISBN)
Unfortunately, price unknown
The article will not be published
Description
Written in a uniform style by a team of experienced authors, this handbook and ready reference represents a practically oriented introduction to the many RNA functions and technologies.
Divided into two sections, the first provides the in-depth background to RNA and its biological functions, while the second part contains hands-on lab protocols of all major RNA technologies. This makes it perfect material for teaching at universities and companies, as well as also serving as an excellent introduction for professionals.
Divided into two sections, the first provides the in-depth background to RNA and its biological functions, while the second part contains hands-on lab protocols of all major RNA technologies. This makes it perfect material for teaching at universities and companies, as well as also serving as an excellent introduction for professionals.
More details
Persons
Günter Kahl is Professor for Plant Molecular Biology at the Biocenter of Johann Wolfgang Goethe-University of Frankfurt am Main, Germany. He currently holds the CSO position with GenXPro GmbH, a company for novel technologies in genomics and transcriptomics, located at the Research Innovation Centre (FIZ) in Frankfurt. After a Ph.D. in plant biochemistry, Dr. Kahl spent two postdoctoral years each at Michigan State University, East Lansing and California Institute of Technology, Pasadena USA). Main research: (1) gene technology, in particular the isolation and characterization of plant defense genes and their promoters, and the use of in vitro modified defense genes for the improvement of plant crops via gene transfer; (2) plant genome analysis, development of molecular marker technologies for genomic fingerprinting, establishment of genetic maps, map-based cloning of agronomically important genes, and extensive pathogen genome sequencing and (3) expression profiling of bacteria, plant, animal and human tissues with expression microarrays and other high-throughput techniques. Author of more than 250 scientific publications. Research supported by the German Research Council (DFG), the European Commission, Stiftung Volkswagenwerk, Fritz-Thyssen-Stiftung, Gesellschaft für Technische Zusammenarbeit (GTZ) and many others. Since most of his research is on crops of subtropical and tropical regions, he cooperates with a series of international and national research institutions in Japan, the USA, France, UK, Germany, Syria, India, Mexico, Spain, Colombia, Venezuela and Chile. Organized molecular marker courses in many countries and served in expert missions for IAEA, FAO and UNESCO.
Carlos Mauricio Molina Medina is a Colombian molecular biologist and bioinformatician, graduated from Frankfurt University with a Ph.D. thesis on "SuperSAGE, a deep transcriptome pprofiling technique" with upmost credits (in German "summa cum laude"). He spent two postdoctoral years in Dijon, France, where he intensively worked on the generation and characterization of plant mutants. He is preparing to leave for a position of an Assistant Professor at the University of Kiel (Germany). Dr. Molina has published a series of peer-reviewed publications and two book articles, and presented seminars on his research in Germany, France, England and the USA. He has extensive training and expertise in bioinformatics.
ErnestinaValadez-Moctezuma is a Mexican biologist graduated from Universidad Nacional Autónoma de Mexico. She is full-time professor at the Universidad Autónoma Chapingo, where heads the molecular biology laboratory. She teaches both graduate and undergraduate courses in plant genetics, molecular biology and biotechnology, especially gene expression, and also conduct research on vegetable crops and plant protection. She has published numerous articles in different journals, some books and book chapters derived from personal and team work studies. Currently she is deeply involved in the area of molecular markers in plant crops and plant pathogens where she significantly contributed to e.g. the Mexican Opuntia genome and Fusarium and virus complex molecular characterization.
Carlos Mauricio Molina Medina is a Colombian molecular biologist and bioinformatician, graduated from Frankfurt University with a Ph.D. thesis on "SuperSAGE, a deep transcriptome pprofiling technique" with upmost credits (in German "summa cum laude"). He spent two postdoctoral years in Dijon, France, where he intensively worked on the generation and characterization of plant mutants. He is preparing to leave for a position of an Assistant Professor at the University of Kiel (Germany). Dr. Molina has published a series of peer-reviewed publications and two book articles, and presented seminars on his research in Germany, France, England and the USA. He has extensive training and expertise in bioinformatics.
ErnestinaValadez-Moctezuma is a Mexican biologist graduated from Universidad Nacional Autónoma de Mexico. She is full-time professor at the Universidad Autónoma Chapingo, where heads the molecular biology laboratory. She teaches both graduate and undergraduate courses in plant genetics, molecular biology and biotechnology, especially gene expression, and also conduct research on vegetable crops and plant protection. She has published numerous articles in different journals, some books and book chapters derived from personal and team work studies. Currently she is deeply involved in the area of molecular markers in plant crops and plant pathogens where she significantly contributed to e.g. the Mexican Opuntia genome and Fusarium and virus complex molecular characterization.
Author
University of Frankfurt, Germany
Unite de Rech. en Legumineuses, Dijon, France
Universidad Auton. de Chapingo, Texcoco Chapingo, Mexico
Content
The RNA World
(Transcripts, Transcriptomes, Transcriptomics)
(Gene Expression Profiling: Theory and Laboratory Protocols)
Günter Kahl, Carlos Molina Medina and Ernestina Valadez-Moctezuma
____________________________________________
Preface
Introduction
Chapter A. The various genetic and epigenetic codes
Recommended Reading
Chapter B. Transcripts and transcriptomes
Recommended Reading
Chapter C. RNA processing, transport, translation and turnover
Recommended Reading
Chapter D. Transcriptomics and its tools
Recommended Reading
Chapter E. The dawn of microarrays: The advancement of tag-based expression platforms
Recommended Reading
Chapter F. The impact of next and next-next-generation sequencing technologies on transcriptomics
Recommended Reading
Chapter G. Next-next transcriptomics: RNA-seq, dRNA-seq and future technologies
Recommended Reading
Chapter H. eQTLs, methQTLs, pQTLs: Links to practice
Recommended Reading
Protocols of selected gene expression technologies
1 Messenger RNA isolation (Precautions and Procedures)
Recommended Reading
2 microRNA isolation
Recommended Reading
3 cDNA synthesis
Recommended Reading
4 Enrichment of target sequences
Recommended Reading
5 Primer development
Recommended Reading
6 qPCR
Recommended Reading
7 deepSuperSAGE (HT-SuperSAGE)
Recommended Reading
8 deepCAGE
Recommended Reading
9 RNA-Seq and dRNA-Seq
Recommended Reading
10 MicroRNA and target messenger RNA: The degradome
Recommended Reading
Web Links
Glossary
Index
(Transcripts, Transcriptomes, Transcriptomics)
(Gene Expression Profiling: Theory and Laboratory Protocols)
Günter Kahl, Carlos Molina Medina and Ernestina Valadez-Moctezuma
____________________________________________
Preface
Introduction
Chapter A. The various genetic and epigenetic codes
Recommended Reading
Chapter B. Transcripts and transcriptomes
Recommended Reading
Chapter C. RNA processing, transport, translation and turnover
Recommended Reading
Chapter D. Transcriptomics and its tools
Recommended Reading
Chapter E. The dawn of microarrays: The advancement of tag-based expression platforms
Recommended Reading
Chapter F. The impact of next and next-next-generation sequencing technologies on transcriptomics
Recommended Reading
Chapter G. Next-next transcriptomics: RNA-seq, dRNA-seq and future technologies
Recommended Reading
Chapter H. eQTLs, methQTLs, pQTLs: Links to practice
Recommended Reading
Protocols of selected gene expression technologies
1 Messenger RNA isolation (Precautions and Procedures)
Recommended Reading
2 microRNA isolation
Recommended Reading
3 cDNA synthesis
Recommended Reading
4 Enrichment of target sequences
Recommended Reading
5 Primer development
Recommended Reading
6 qPCR
Recommended Reading
7 deepSuperSAGE (HT-SuperSAGE)
Recommended Reading
8 deepCAGE
Recommended Reading
9 RNA-Seq and dRNA-Seq
Recommended Reading
10 MicroRNA and target messenger RNA: The degradome
Recommended Reading
Web Links
Glossary
Index