Immunochemistry in Practice
3rd Edition
Published on 18. April 1996
Book
Paperback/Softback
384 pages
978-0-86542-633-7 (ISBN)
Description
This practical book provides scientists of all disciplines with clear descriptions of the plethora of immunochemical techniques available for detecting, isolating and characterising biological molecules. The revolution in molecular biology and biotechnology in the past decade has meant that immunochemistry has become an integral part of the experimental armoury of scientists from a variety of backgrounds. At the heart of this technology is the remarkable specificity that antibodies display toward their epitopes. The specific recognition offered by immunological molecules has resulted in their uptake for a wide range of purposes. This book explains how to produce and purify antibodies and how to tag them for later visualisation.
More details
Edition
3rd Revised edition
Language
English
Place of publication
Oxford
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Edition type
Revised edition
Illustrations
83
Dimensions
Height: 243 mm
Width: 173 mm
Thickness: 21 mm
Weight
838 gr
ISBN-13
978-0-86542-633-7 (9780865426337)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
1. Basic techniques; 2. Production of antibodies; 3. Purification of immunoglobulins, constituent chains and fragments; 4. Isolation and fractionation of lymphocytes; 5. Radiolabelling techniques; 6. Precipitation techniques in agar and agarose; 7. Analytical electrophoresis and isoelectric focusing; 8. Specific detection of antigens separated by polyacrylamide gel electrophoresis; 9. Preparative gel techniques; 10. Affinity chromatography and immunoprecipitation; 11. Coupling antibodies to fluorochromes, enzymes and biotin; 12. Immunoassays; 13. Immunocytochemistry, immunohistochemistry and flow cytofluorimetry; Appendix 1: Abbreviations; Appendix 2: Buffers; Appendix 3: Manufacturers and suppliers; References; Index