Immunochemistry in Practice
Blackwell Science Ltd (Publisher)
Published on 21. September 1987
Book
Paperback/Softback
318 pages
978-0-632-01723-2 (ISBN)
Description
The purpose of this practical book is to provide scientists, whatever their specialty, with an account of the various techniques available for detecting, estimating and isolating biological molecules. The methods presented are based on the range of immune reactions that have been discovered in recent years, the usefulness of which has quickly been put to work in many research programmes, as well as the biotechnological industries. At the forefront of the advances made, has been an improved understanding of the role of monoclonal antibodies. Each chapter in the second edition of "Immunochemistry in Practice" has been updated to take these advances into consideration. Clear guidance on purifying, digesting and applying fractions has been given along with a wide coverage of immunohistochemistry and immunoblotting. The sections on the immunoradiometric assays, ELISA and IRMA, have also been expanded in this edition.
More details
Edition
New edition
Language
English
Place of publication
Oxford
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
Professional and scholarly
Edition type
New edition
Illustrations
77 illustrations
Dimensions
Height: 230 mm
Width: 150 mm
ISBN-13
978-0-632-01723-2 (9780632017232)
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Schweitzer Classification
Content
Basic techniques; production of antibodies; purification of immunoglobulins, constituent chains and fragments; isolation and fractionation of lymphocytes; radiolabelling techniques; precipitation techniques in agar and agarose; polyacrylamide gel techniques; specific detection of antigens separated by polyacrylamide gel electrophoresis; preparative gel techniques; affinity chromatography and immunoprecipitation; immunoassays; immunocytochemistry and immunohistochemistry.