Laboratory Experiments in Microbiology
6th Edition
Published on 1. May 2001
Book
Spiral bound
440 pages
978-0-8053-7589-3 (ISBN)
Description
Newly revised to accompany Microbiology: An Introduction, Seventh Edition by Tortora, Funke, and Case, this lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology.
Intended as a manual of basic microbiologic techniques, this popular lab manual features applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various preprofessional programs. Experiments have been refined in this new edition to encourage students to develop critical thinking skills as well as learn basic facts and technical skills. Material with direct application to clinical and commercial labs is included whenever possible, and increased emphasis is placed on lab safety.
Intended as a manual of basic microbiologic techniques, this popular lab manual features applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various preprofessional programs. Experiments have been refined in this new edition to encourage students to develop critical thinking skills as well as learn basic facts and technical skills. Material with direct application to clinical and commercial labs is included whenever possible, and increased emphasis is placed on lab safety.
More details
Edition
6th edition
Language
English
Place of publication
Upper Saddle River
United States
Target group
Professional and scholarly
Dimensions
Height: 275 mm
Width: 233 mm
Thickness: 16 mm
Weight
970 gr
ISBN-13
978-0-8053-7589-3 (9780805375893)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
Book
05/2003
7th Edition
Pearson Education (US)
€86.84
Article exhausted; check for reprint
Previous edition
Ted R. Johnson | Christine L. Case
Laboratory Experiments in Microbiology Lab Manual
Book
05/1998
5th Edition
Pearson Education (US)
€60.84
Article exhausted; check for reprint
Persons
Ted R. Johnson is a biology professor at St. Olaf College, where he has been teaching for 20 years. He earned a B.A. from Augsburg College and an M.A., and Ph.D. from the University of Illinois at Chicago. He teaches courses in microbiology, immunology, and cancer biology and his researchinterests include cancer biology, immunology, aging, and soil microbiology.
Christine L. Case is a registered microbiologist and professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 25 years. In addition to teaching and affiliations with numerous scientific organizations, Chris contributes regularly to professional literature, develops innovative instructional methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. She is an avid photographer and many of her photographs appear in Microbiology: An Introduction.
Christine L. Case is a registered microbiologist and professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 25 years. In addition to teaching and affiliations with numerous scientific organizations, Chris contributes regularly to professional literature, develops innovative instructional methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. She is an avid photographer and many of her photographs appear in Microbiology: An Introduction.
Content
Introduction.
I. MICROSCOPY.
1. Use and Care of the Microscope.
2. Examination of Living Microorganisms.
II. STAINING METHODS.
3. Preparation of Smears and Simple Staining.
4. Negative Staining.
5. Gram Staining.
6. Acid-fast Staining.
7. Structural Stains (Endospore, Capsule, and Flagella).
8. Morphologic Unknown.
III. CULTIVATION OF BACTERIA.
9. Microbes in the Environment.
10. Transfer of Bacteria: Aseptic Technique.
11. Isolation of Bacteria by Dilution Techniques.
12. Special Media for Isolating Bacteria.
IV. MICROBIAL METABOLISM.
13. Carbohydrate Catabolism.
14. Fermentation of Carbohydrates.
15. Protein Catabolism, Part 1.
16. Protein Catabolism, Part 2.
17. Respiration.
18. Rapid Identification Methods.
V. MICROBIAL GROWTH.
19. Oxygen and the Growth of Bacteria.
20. Determination of a Bacterial Growth Curve: The Role of Temperature.
21. Other Influences on Microbial Growth: Osmotic Pressure and pH.
VI. CONTROL OF MICROBIAL GROWTH.
22. Physical Methods of Control: Heat.
23. Physical Methods of Control: Ultraviolet Radiation.
24. Chemical Methods of Control: Disinfectants and Antiseptics.
25. Chemical Methods of Control: Antimicrobial Drugs.
26. Effectiveness of Hand Scrubbing.
VII. MICROBIAL GENETICS.
27. Regulation of Gene Expression.
28. Isolation of Bacterial Mutants.
29. Transformation of Bacteria.
30. DNA Fingerprinting.
31. Genetic Engineering.
32. Ames Test for Detecting Possible Chemical Carcinogens.
VIII. THE MICROBIAL WORLD.
33. Unknown Identification and Bergey's Manual.
34. Fungi: Yeasts.
35. Fungi: Molds.
36. Phototrophs: Algae and Cyanobacteria.
37. Protozoa.
IX. VIRUSES.
38. Isolation and Titration of Bacteriophages.
39. Plant Viruses.
X: INTERACTION OF MICROBE AND HOST.
40. Epidemiology.
41. Koch's Postulates.
XI. IMMUNOLOGY.
42. Nonspecific Resistance.
43. Blood Group Determination: Slide Agglutination.
44. Agglutination Reactions: Microtiter Agglutination.
45. ELISA Technique.
XII. MICROORGANISMS AND DISEASE.
46. Bacteria of the Skin.
47. Bacteria of the Respiratory Tract.
48. Bacteria of the Mouth.
49. Bacteria of the Gastrointestinal Tract.
50. Bacteria of the Urogenital Tract.
51. Identification of an Unknown from a Clinical Sample.
XIII. MICROBIOLOGY AND THE ENVIRONMENT.
52. Microbes in Water: Multiple-Tube Technique.
53. Microbes in Water: Membrane Filter Technique.
54. Microbes in Food: Contamination.
55. Microbes Used in the Production of Foods.
56. Microbes in the Soil: The Nitrogen and Sulfur Cycles.
57. Microbes in Soil: Bioremediation.
APPENDICES.
A. Pipetting.
B. Dilution Techniques and Calculations.
C. Use of the Spectrophotometer.
D. Graphing.
E. Use of the Dissecting Microscope.
F. Use of the Membrane Filter.
G. Electrophoresis.
H. Keys to Bacteria.
Index.
I. MICROSCOPY.
1. Use and Care of the Microscope.
2. Examination of Living Microorganisms.
II. STAINING METHODS.
3. Preparation of Smears and Simple Staining.
4. Negative Staining.
5. Gram Staining.
6. Acid-fast Staining.
7. Structural Stains (Endospore, Capsule, and Flagella).
8. Morphologic Unknown.
III. CULTIVATION OF BACTERIA.
9. Microbes in the Environment.
10. Transfer of Bacteria: Aseptic Technique.
11. Isolation of Bacteria by Dilution Techniques.
12. Special Media for Isolating Bacteria.
IV. MICROBIAL METABOLISM.
13. Carbohydrate Catabolism.
14. Fermentation of Carbohydrates.
15. Protein Catabolism, Part 1.
16. Protein Catabolism, Part 2.
17. Respiration.
18. Rapid Identification Methods.
V. MICROBIAL GROWTH.
19. Oxygen and the Growth of Bacteria.
20. Determination of a Bacterial Growth Curve: The Role of Temperature.
21. Other Influences on Microbial Growth: Osmotic Pressure and pH.
VI. CONTROL OF MICROBIAL GROWTH.
22. Physical Methods of Control: Heat.
23. Physical Methods of Control: Ultraviolet Radiation.
24. Chemical Methods of Control: Disinfectants and Antiseptics.
25. Chemical Methods of Control: Antimicrobial Drugs.
26. Effectiveness of Hand Scrubbing.
VII. MICROBIAL GENETICS.
27. Regulation of Gene Expression.
28. Isolation of Bacterial Mutants.
29. Transformation of Bacteria.
30. DNA Fingerprinting.
31. Genetic Engineering.
32. Ames Test for Detecting Possible Chemical Carcinogens.
VIII. THE MICROBIAL WORLD.
33. Unknown Identification and Bergey's Manual.
34. Fungi: Yeasts.
35. Fungi: Molds.
36. Phototrophs: Algae and Cyanobacteria.
37. Protozoa.
IX. VIRUSES.
38. Isolation and Titration of Bacteriophages.
39. Plant Viruses.
X: INTERACTION OF MICROBE AND HOST.
40. Epidemiology.
41. Koch's Postulates.
XI. IMMUNOLOGY.
42. Nonspecific Resistance.
43. Blood Group Determination: Slide Agglutination.
44. Agglutination Reactions: Microtiter Agglutination.
45. ELISA Technique.
XII. MICROORGANISMS AND DISEASE.
46. Bacteria of the Skin.
47. Bacteria of the Respiratory Tract.
48. Bacteria of the Mouth.
49. Bacteria of the Gastrointestinal Tract.
50. Bacteria of the Urogenital Tract.
51. Identification of an Unknown from a Clinical Sample.
XIII. MICROBIOLOGY AND THE ENVIRONMENT.
52. Microbes in Water: Multiple-Tube Technique.
53. Microbes in Water: Membrane Filter Technique.
54. Microbes in Food: Contamination.
55. Microbes Used in the Production of Foods.
56. Microbes in the Soil: The Nitrogen and Sulfur Cycles.
57. Microbes in Soil: Bioremediation.
APPENDICES.
A. Pipetting.
B. Dilution Techniques and Calculations.
C. Use of the Spectrophotometer.
D. Graphing.
E. Use of the Dissecting Microscope.
F. Use of the Membrane Filter.
G. Electrophoresis.
H. Keys to Bacteria.
Index.