
Cryo-EM Part A: Sample Preparation and Data Collection: Volume 481
Grant Jensen(Editor)
Academic Press
Published on 22. November 2010
Book
Hardback
464 pages
978-0-12-374906-2 (ISBN)
Description
Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
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Dimensions
Height: 229 mm
Width: 152 mm
Weight
880 gr
ISBN-13
978-0-12-374906-2 (9780123749062)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions

Grant Jensen
Cryo-Em Part a
Sample Preparation and Data Collection: Sample Preparation and Data Collection
E-Book
05/2014
Academic Press
€146.00
Available for download
Content
Historical perspective
3D reconstruction from electron micrographs: a personal account of its development
David DeRosier
Preparation of 2-D crystals
Henning Stahlberg
Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie
Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen
A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski
GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark
Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark
Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang
Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky
Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld
How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun
Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng
Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat
Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Juergen M. Plitzkod, Cindi L. Schwartz
Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama
Radiation damage in electron cryomicroscopy
John L. Rubinstein, Lindsay A. Baker
3D reconstruction from electron micrographs: a personal account of its development
David DeRosier
Preparation of 2-D crystals
Henning Stahlberg
Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie
Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen
A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski
GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark
Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark
Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang
Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky
Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld
How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun
Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng
Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat
Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Juergen M. Plitzkod, Cindi L. Schwartz
Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama
Radiation damage in electron cryomicroscopy
John L. Rubinstein, Lindsay A. Baker