
Animal Cell Biotechnology
Methods and Protocols
Nigel Jenkins(Author)
Humana Press Inc.
Published on 13. February 2010
Book
Hardback
XIV, 302 pages
978-0-89603-547-8 (ISBN)
Article exhausted; check for reprint
Description
Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.
Reviews / Votes
A specific feature of cell culture technology is described in each chapter -- such as setting up a cell culture lab, production of large quantities of animal cells and cell products, large scale transfection protocols, and screening cultures for possible viral infection. There is an adequate number of illustrations to help the reader understand the nature of the equipment described and the expected data one should obtain. This book will be useful to individuals interested in the industrial or commercial applications and uses of animal cell culture methods. - Doody's Health Sciences Book Review Journal Animal Cell Biotechnology brings together techniques used in both industry and research laboratories that are focused on the expression of recombinant proteins and other commercial uses of animal cells. The book is divided into five parts describing techniques relevant for each step in this process. Detailed protocols are provided including recipes for culture media. In the last chapter of Part I, the problem of virus contamination of cell cultures used for the manufacture of therapeutic reagents is addressed and the subject is broadened to encompass viruses in both humans, laboratory animals and livestock. Overall, Part V very adequately prepares the reader for the next step which covers the molecular methods of the biotechnology. Part II Molecular methods', comprises six excellent chapters on gene expression optimization, immortalization strategies, production of monoclonal antibodies, cell bank preparation and cell type characterization, including cytogenetic characterization and DNA fingerprinting. - FEBS LettersMore details
Series
Language
English
Place of publication
NJ
United States
Target group
College/higher education
Professional and scholarly
Product notice
Laminated cover
Illustrations
43
43 s/w Abbildungen
Illustrations
Dimensions
Height: 23.5 cm
Width: 15.5 cm
Thickness: 19 mm
Weight
1 gr
ISBN-13
978-0-89603-547-8 (9780896035478)
DOI
10.1385/0896035476
Schweitzer Classification
Other editions
New editions

Book
04/2007
2nd Edition
Humana Press Inc.
€160.49
Shipment within 15-20 days
Content
Immortalization Strategies for Mammalian Cells. Culturing Animal Cells in Fluidized Bed Reactors. Viral Evaluation of Animal Cell Lines Used In Biotechnology. Monitoring Animal Cell Growth and Productivity by Flow Cytometry. Adaptation of Mammalian Cells Grown in Serum Free Medium.Cytotoxicity Testing Using Cell Lines. Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells.Hematopoietic Cells for Cellular and Gene Therapy: I. Basic Assay Techniques. Measurement of Cell-Culture Glycosidase Activity. Cytogenetic Characterization of Recombinant Cells. Hematopoietic Cells for Cellular and Gene Therapy: II. Expansion Protocols.Control of Proteolysis in Cell Culture. Measurement of Cell Death in Culture. GPI-Anchored Fusion Proteins. Harvesting GPI-Anchored Proteins From CHO Cells. Optimizing Gene Expression in Mammalian Cells.