NanoCellBiology of Secretion
Imaging Its Cellular and Molecular Underpinnings
Published on 19. January 2012
Book
Paperback/Softback
XII, 70 pages
978-1-4614-2437-6 (ISBN)
Description
Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Over the past 15 years, there has been a renewed understanding of living cells at the molecular level. Atomic Force Microscopy, Laser Force Microscopy, single secretory vesicle patch clamp studies, highresolution electron microscopy, and x-ray diffraction, are some of the tools now being used to unravel the intricacies of a living cell at the molecular level. Opening with an explanation of Materials and Methods, NanoCellBiology then moves through discussions of porosome discovery, calcium and SNARE-induced fusion, and vesicle swelling before winding up in a final chapter of conclusions and future studies. Succinctly packaged as SpringerBrief, this book is a must for those studying or conducting research in cell biology, biochemistry or nanobiology/nanotechnology. This book will be invaluable to faculty & graduate students involved in Nano Courses; Cell Biology Courses; Biophysics Courses; and Biochemistry Courses as well as practicing Cell Biologists, Biochemists and BioPhysicists.
Reviews / Votes
"This is a reference discussing the molecular mechanisms of the following problems: Exocytocis, secretion of hormones in pancreatic and excitable cells. . This reference is of use to physiologists, biochemists, cell biologists, and membrane experts." (Joseph J. Grenier, Amazon.com, September, 2015)
More details
Series
Edition
2012
Language
English
Place of publication
New York
United States
Target group
Professional and scholarly
Professional/practitioner
Illustrations
5 s/w Abbildungen, 33 farbige Abbildungen
XII, 70 p. 38 illus., 33 illus. in color.
Dimensions
Height: 235 mm
Width: 155 mm
Thickness: 6 mm
Weight
143 gr
ISBN-13
978-1-4614-2437-6 (9781461424376)
DOI
10.1007/978-1-4614-2438-3
Schweitzer Classification
Other editions
Additional editions
E-Book
01/2012
1st Edition
Springer
€53.49
Available for download
Content
Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Over the past 15 years, there has been a renewed understanding of living cells at the molecular level. Atomic Force Microscopy, Laser Force Microscopy, single secretory vesicle patch clamp studies, highresolution electron microscopy, and x-ray diffraction, are some of the tools now being used to unravel the intricacies of a living cell at the molecular level. Opening with an explanation of Materials and Methods, NanoCellBiology then moves through discussions of porosome discovery, calcium and SNARE-induced fusion, and vesicle swelling before winding up in a final chapter of conclusions and future studies. Succinctly packaged as SpringerBrief, this book is a must for those studying or conducting research in cell biology, biochemistry or nanobiology/nanotechnology. This book will be invaluable to faculty & graduate students involved in Nano Courses; Cell Biology Courses; Biophysics Courses; and Biochemistry Courses as well as practicing Cell Biologists, Biochemists and BioPhysicists.Table of contetns: Introduction.- Materials & Methods.- Porosome Discovery.- Calcium & SNARE-Induced Membrane Fusion.- SNAREs need to reside in opposing membrane.- Membrane curvature dictates SNARE size.- Disassembly of membrane-associated SNARE complex.- CD spectroscopy confirm membrane requirement for t-/v-SNARE assembly.- SNAREs bring opposing bilayers closer for calcium bridging.- Membrane lipids influence SNARE complex assembly-disassembly.- Vesicle swelling and content expulsion during cell secretion.- Secretory vesicle swelling is required for intravesicular content expulsion.- Molecular mechanism of secretory vesicle swelling.- Presence of beta receptors at the secretory vesicle membrane.- Conclusion and future studies.