This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis.
Authoritative and cutting-edge,
CRISPR-Cas Methods, Volume 2
aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.
The chapter "CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Series
Edition
Language
Place of publication
Publishing group
Springer-Verlag New York Inc.
Target group
Professional and scholarly
Illustrations
12 s/w Abbildungen, 106 farbige Abbildungen
XVII, 397 p. 118 illus., 106 illus. in color.
Dimensions
Height: 260 mm
Width: 183 mm
Thickness: 29 mm
Weight
ISBN-13
978-1-0716-1656-7 (9781071616567)
DOI
10.1007/978-1-0716-1657-4
Schweitzer Classification
A Complete Methodology for the Instruction of CRISPR-based Gene Editing Using a Simplified Cell-free Extract System with Genetic Readout in Bacteria.-
In silico
Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing.- Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants.- CRISPR-dCas9 Based Targeted Manipulation of DNA Methylation in Plants.- Rapid Assembly of Multiplex Natural CRISPR Arrays.- Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants.- Designing, Performing and Analysing CRISPR-Cas9-mediated Genome Editing Experiments in Leguminous Plant.- Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9.- Targeted Base Editing in Soybean using a CRISPR-Cas9 Cytidine Deaminase Fusion.- Genome Editing in Potato Using a Hairy Root Transformation System.- Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato.- CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single and Multi-Target Vector Construction.- Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9.- CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants.- CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells.- Cell-Type-Specific CRISPR-Cas9 System with miRNAs.- CRISPR-Cas9 gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-puro Vectors.- An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA that Regulate CRISPR-Cas systems.- Genetic Engineering of a Phage-based Delivery System for Endogenous III-A CRISPR-Cas System against
Mycobacterium tuberculosis.-
CRISPR-Cas9-Mediated Genome Editing in
Escherichia coli
Bacteriophages.- CRISPR-Cas Genome Editing in the Cellulolytic Bacterium
Clostridium thermocellum.-
Gene Targeting in
Caenorhabditis elegans
Using a Combination of Multiple sgRNAs and a Homologous Recombination-mediated Repair.- Design of Repair Templates for CRISPR-Cas9-triggered Homologous Recombination in
Caenorhabditis elegans.
