Bone Research Protocols

Isolation and Generation of Osteoclasts.- Isolation and Generation of Osteoblasts.- Isolation, Purification, Generation, and Culture of Osteocytes.- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo.- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies.- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation.- The ex vivo Organ Culture of Bone.- Ex-Vivo Model Systems of Cancer-bone Cell Interactions.- Mechanical Properties of Bone ex vivo.- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation.- Analysis of Transcriptional Regulation in Bone Cells.- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization.- Ovariectomy/Orchiectomy in Rodents.- In Vivo Models of Rheumatoid Arthritis.- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus.- Models of Prostate Cancer Bone Metastasis.-Animal Models of Breast Cancer Bone Metastasis.- Murine Models of Bone Sarcomas.- Mouse Models of Melanoma Bone Metastasis.- Animal Models of Multiple Myeloma.- The Calvarial Injection Assay.- In Vivo Models of Mechanical Loading.- Animal Models for the Study of Bone Derived Pain.- Histomorphometry in Rodents.- Immunostaining of Skeletal Tissues.- Techniques for the Study of Apoptosis in Bone.- Live Cell Imaging of Bone Cell and Organ Cultures.- Analysis of Bone Architecture in Rodents using micro Computed Tomography.- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents.- Quantitative X-ray Imaging of Mouse Bone by Faxitron.- Scanning Electron Microscopy of Bone.- Transmission Electron Microscopy of Bone.- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy.- Fourier Transform Infrared Imaging of bone.- Raman Microscopy and Bone.