Biomass Conversion
Description
Biomass conversion research is a combination of basic science, applied science, and engineering testing and analysis. Conversion science includes the initial treatment (called pre-treatment) of the feedstock to render it more amenable to enzyme action, enzymatic saccharification, and finally product formation by microbiological or chemical processes. In Biomass Conversion: Methods and Protocols, expert researchers in the field detail methods which are now commonly used to study biomass conversion. These methods include Biomass Feedstocks and Cellulose, Plant Cell Wall Degrading Enzymes and Microorganisms, and Lignins and Hemicelluloses. Written in the highly successful Methods in Molecular Biology T series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting informed, reproducible results in the laboratory.
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Content
Challenges for Assessing the Performance of Biomass Degrading Biocatalysts.-Overview of Computer Modeling of Cellulose.-Imaging Cellulose Using Atomic Force Microscopy.-Preservation and Preparation of Lignocellulosic Biomass Samples for Multi-scale Microscopy Analysis.-Coherent Raman Microscopy Analysis of Plant Cell Walls.-Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling.-Immunological Approaches To Plant Cell Wall and Biomass Characterization: Immunolocalization of Glycan Epitopes.-A Method to Evaluate Biomass Accessibility in Wet State Based on Thermoporometry.-Cellulase Processivity.-A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Insoluble Polysaccharide Substrates.-Bacterial Cadherin Domains as Carbohydrate Binding Modules: Determination of Affinity Constants to Insoluble Complex Polysaccharides.-Affinity Electrophoresis as a Method for Determining Substrate-Binding Specificity of Carbohydrate-Active Enzymes for Soluble Polysaccharides.-Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence Microscopy.-Bioprospecting Metagenomics for New Glycoside Hydrolases.-Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates.-Assessing the Protein Concentration in Commercial Enzyme Preparations.-Reducing the Effect of Variable Starch Levels in Biomass Recalcitrance Screening.-Analysis of Transgenic Glycoside Hydrolases Expressed in Plants: T. reesei CBH I and A. cellulolyticus EI.-Structural Characterization of the Heteroxylans from Poplar and Switchgrass.-Laser Microdissection and Genetic Manipulation Technologies to Probe Lignin Heterogeneityand Configuration in Plant Cell Walls.-Lignin-Degrading Enzyme Activities.