Biocatalyst Design for Stability and Specificity

Part 1 Protein stability: effects of mutations on thermodynamic properties or proteins, Julian M. Sturtevant; contribution of hydrogen bonding and the hydrophobic effect to conformational stability of ribonuclease Tl, C. Nick Pace et al; protein structure and stability assessment by circular dichroism spectroscopy, Mark C. Manning; structure-function relationship of hyperthermophilic enzymes, Rainer Jaenicke; psychrophilic proteinases from Atlantic cod, J.B. Bjarnason et al; thermal and pH stress in thermal denaturation of trichoderman research cellobiohydrolase - supporting evidence for a two-transition model, John Baker and Michael E. Himmel; recombinant protein stabilization through engineered metal-chelating sites, Pablo Umaiia et al; engineering nonaqueous solvent-compatible enzymes, Frances H. Arnold et al. Protein folding: mutational effects on inclusion body formation, Ronald Wetzet and Boris A. Chrunyk; characterization and refolding of b-lactamase inclusion bodies in escheria coli, Pascal Valax and George Georgiou; participation of GroE heat shock proteins in polypeptide folding, Anthony A. Gatenby et al; cosolvent effects on refolding and aggregation, Jeffrey L. Cleland and Daniel I.C. Wang; facilitation of protein folding and the reversibility of denaturation, P.M. Horowitz. Part 3 Multifunctional proteins: artificial bifunctional enzymes - a tool to improve consecutive enzyme reactions and cell metabolism, Leif Bdlow; proteins designed for adherence to cellulose, Edgar Ong et al; modification of regulatory communication in aspartate transcarbamoylase, M.E. Wales et al; C-tetrahydrofolate synthase - dissection of active site and domain structure by protein engineering, Anice E. Tbigpen et al. Part 4 Design of cellulases by recombinant methods: properties of native and site-mutagenized cellobiohydrolase, C. Barnett et al; recombinant, beta-glucosidase of trichoderma reesei, Tim Fowler; structure-function relationships in cellulase genes, David B. Wilson; clostridium thermocellum cellulosome - new mechanistic concept for cellulose degradation, J.H. David Wu. Part 5 Improving natural enzymes by chemical cross-linking: protein chemical cross-linking - implications for protein stabilization, Shan S. Wong et al; glutaraldehyde cross-linking - fast and slow modes, Timothy J.A. Johnson.