RNA Processing: v. 1
Stephen J. Higgins(Author)
Oxford University Press
Published on 1. February 1994
Book
Spiral bound
232 pages
978-0-19-963344-9 (ISBN)
Description
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
halftones, line figures, tables, bibliography
ISBN-13
978-0-19-963344-9 (9780199633449)
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Schweitzer Classification
Persons
Content
Synthesis and purification of RNA substrates; Characterization of RNA; Splicing of mRNA precursors in mammalian cells; Isolation and characterization of ribonucleoprotein complexes; Analysis of ribonucleoprotein interactions; Analysis of pre-mRNA splicing in yeast; Appendix