Mammalian Cell Biotechnology in Protein Production

Part 1 Genetic manipulation of mammalian cells: Heterologous expression of genes in mammalian cells, Hauser; Permanent gene expression in mammalian cells - gene transfer and selection, Schlokar; Vector for gene transfer and expression in animal cells, Sandig et al; Aspects of gene transfer and gene amplification in recombinant mammalian cells, Wurm; Isolation of recombinant cell clones exhibiting high-level expression of the introduced gene, Wirth; Genetic engineering of antibodies and derivatives from mammalian cells,Weidle; Safety evaluation of products derived from mammalian cell lines, Onions. Part 2 Biological aspects of animal cells: Metabolic control of animal cell culture processes, Wagner; Glycosylation - a post-translational modification, Savage. Part 3 Cell cultivation technology: Bioreactors designed for animal cells, Tokashiki, Yokoyama; Hydrodinamic properties in bioreactors, Chattopadhyay et al; Kinetics and simulation of animal cell processes, Goergen et al; On-line and Off-line process analysis, Scheper et al. Part 4 Down-stream processing: Principles of product extraction from cell culture and purification for pharmaceutical proteins, Walter, Allgaier.