Spectrophotometry and Spectrofluorimetry

This volume is intended to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day-to-day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications. These include spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phoshorescence studies, circular dichroism and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability.