Redox-Mediated Signal Transduction
Description
Cutting-edge and informative, Redox-Mediated Signal Transduction: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in the evolving redox field.
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Content
The Role of Redox in Signal Transduction.- Methods for the Addition of Redox Compounds.- Investigating ROS, RNS, and H2S Sensitive Signalling Proteins.- Measurement of 4-hydroxynonenal (4-HNE) Protein Adducts by ELISA.- Using Flow Cytometry to Detect and Measure Intracellular Thiol Redox Status in Viable T Cells from Heterogeneous Populations.- Detection of S-Nitrosation and S-Glutathionylation of the Human Branched-Chain Aminotransferase Proteins.- Visualization of Intracellular Hydrogen Peroxide produced by DAO with Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells.- Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy to the Study of Oxidative Stress in Biological Systems.- Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins.- Working with Hypoxia.- Predicting the Effects of Low Dose-Rate Ionising Radiation on Redox Potential in Plant Cells.- Thioredoxin-1 PEGyletion as an In Vitro Method for Drug Target Identification.- Redox-Regulated, Targeted Affinity Isolation of NADH-Dependent Protein Interactions with the Branched Chain Aminotransferase Proteins.- Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures.- Equations to Support Redox Experimentation.