PCR: Methodology v. 2
B. D. Hames(Author)
Oxford University Press
Published on 1. June 1995
Book
Hardback
358 pages
978-0-19-963425-5 (ISBN)
Description
PCR is now one of the most widely used basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications. This volume sets out to address some of the new applications of PCR, including direct sequencing, mRNA quantitation and expression, genomic DNA mapping and mutation analysis.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
line drawings
ISBN-13
978-0-19-963425-5 (9780199634255)
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Schweitzer Classification
Persons
Content
Optimizing PCR; Use of specialty phosphoramidites in PCR; Chemical methods for 5' non-isotopic labelling of PCR probes and primers; Solid-phase PCR; Solid-phase sequencing of PCR products; cDNA cloning by RT - PCR; Quantification of DNA and RNA by PCR; PCR MIMICS: competitive DNA fragments for use in quantitative PCR; In vitro expression of proteins from PCR products; PCR-based approaches to human genome mapping; Fingerprinting of DNA and RNA using arbitrarily primed PCR; Mutational analysis; Mutational analysis: new mutations; Linear amplification for the in vitro study of ligand/DNA interactions; Ligand-mediated PCR.