Gene Probes
Published on 1. December 1995
Book
Hardback
732 pages
978-0-19-963613-6 (ISBN)
Description
The preparation of gene probes is central to much of molecular biology, creating essential tools for genetic research, and gene probes have a wide range of applications. This two-volume set covers the preparation of such probes and their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. It is hoped that the two books will contribute to a growing field of research.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
7 halftones, 24 line figures in Vol. 1: 37 halftones, 24 line figures in Vol. 2, bibliography
ISBN-13
978-0-19-963613-6 (9780199636136)
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Schweitzer Classification
Persons
Content
Volume 1: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of synthetic oligodeoxynucleotide probes; Probe preparation of PCR; Synthesis of labelled riboprobes; Antibody probes. Volume 2: Hybridization strategy; Screening recombinant DNA libraries by hybridization and amplification; Screening human recombinant libraries with nucleic acid probes; Antibody screening of bacterial expression systems; Isolation of cDNA clones by direct expression in mammalian cells; Genomic mapping and gene structure analysis; Analysis of gene expression by blotting techniques; In situ hybridization of nucleic acid probes to cellular RNA; Applications of chromosomal in situ hybridization; Analysis of gene expression analysis by PCR: a quantitative approach; Analysis of protein expression by immunocytochemistry; Gene targeting in mammalian cells; Directed mutagenesis and mutant analysis; Human gene mutational analysis.