Gene Probes

Volume 1 (Preparation). Introduction. 1.: Strategies for using gene probes. 2.: Preparation of nucleic acids for use as probes. 3.: DNA radiolabelling and detection. 4.: Non-radioactive labelling of DNA probes. 5.: Preparation of synthetic oligodeoxynucleotide probes. 6.: Probe preparation of PCR. 7.: Synthesis of labelled riboprobes. 8.: Antibody probes. Appendix 1. Appendix 2. Volume 2 (Applications). 1.: Hyrbidization strategy. 2.: Screening recombinant DNA libraries by hybriization and amplification. 3.: Screening human recombinant libraries with nucleic acid probes. 4.: Antibody screening of bacterial expression systems. 5.: Isolation of cDNA clones by direct expression in mammalian cells. 6.: Genomic mapping and gene structure analysis. 7.: Analysis of gene expression by blotting techniques. 8.: In situ hybridization of nucleic acid probes to cellular RNA. 9.: Applications of chromosomal in situ hybridization. 10.: Analysis of gene expression analysis by PCR: a quantitative approach. 11.: Analysis of protein expression by immunocytochemistry. 12.: Gene targeting in mammalian cells. 13.: Directed mutageneis and mutant analysis. 14.: Human gene mutational analysis. Appendix 1. Appendix 2