Gene Probes 2
A Practical Approach
Published on 23. November 1995
Book
Paperback/Softback
446 pages
978-0-19-963414-9 (ISBN)
Description
Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. A Companion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in
importance.
importance.
More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Illustrations
halftones, line figures, tables
Dimensions
Height: 234 mm
Width: 156 mm
Thickness: 23 mm
Weight
687 gr
ISBN-13
978-0-19-963414-9 (9780199634149)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
1. Hybridization strategy ; 2. Screening recombinant DNA libraries by hybridization and amplification ; 3. Screening human recombinant libraries with nucleic acid probes ; 4. Antibody screening of bacterial expression systems ; 5. Isolation of cDNA clones by direct expression in mammalian cells ; 6. Genomic mapping and gene structure analysis ; 7. Analysis of gene expression by blotting techniques ; 8. In situ hybridization of nucleic acid probes to cellular RNA ; 9. Applications of chromosomal in situ hybridization ; 10. Analysis of gene expression analysis by PCR: a quantitative approach ; 11. Analysis of protein expression by immunocytochemistry ; 12. Gene targeting in mammalian cells ; 13. Directed mutagenesis and mutant analysis ; 14. Human gene mutational analysis ; Appendix 1 ; Appendix 2