Subcellular Fractionation
Published on 1. March 1997
Book
Hardback
359 pages
978-0-19-963495-8 (ISBN)
Description
Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers many necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
13 halftones, 33 line figures, bibliography
ISBN-13
978-0-19-963495-8 (9780199634958)
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Schweitzer Classification
Persons
Content
1.: Homogenization of tissues and cells. 2.: Isolation of subcellular fragments. 3.: Isolation and characterization of nuclei and nuclear subfractions. 4.: Subcellular fractionation of mitochondria. 5.: Isolation and characterization of peroxisomes. 6.: Lysosomes and endocytosis. 7.: The membranes of the secretory and exocytic pathways. 8.: Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells. 9.: Ribosomes and polysomes. 10.: Electron microscopy of organelles