DNA Sequencing Protocols

DNA SEQUENCING PROTOCOLS, Second Edition Edited by Colin A. Graham and Alison J. M. Hill Contents 1. Introduction to DNA sequencing Colin A. Graham and Alison J. M. Hill 2. M13 cloning Joachim Messing 3. M13 sequencing Qingzhong Yu 4. Primer Design and Primer directed sequencing Ulrike Gerischer and Peter Dürre 5. Direct sequencing of DNA produced in a Polymerase Chain Reactio Ulrike Gerischer 6. Solid phase fluorescent sequencing of the CFTR gene Harry Cuppens and Jean-Jacques Cassiman 7. Shotgun sequencing Alan T. Bankier 8. Cycle sequencing Robert W. Blakesley 9. Cycle Sequencing of PCR-Amplified Genomic DNA with Dye-Labelled Universal Primers Johannes Zschocke and Georg F. Hoffmann 10. Automated fluorescent DNA sequencing on the ABI PRISM 377 Jonathan R. E. MacBeath, Suzanna S. Harvey, and Nicola J. Oldroyd 11. Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic analyser David Watts and Jonathan R. E. MacBeath 12. Fluorescent sequencing protocols for the ALF Shirley MacQuaid 13. Fluorescent sequencing for heterozygote mutation detection Colin A. Graham and Alison J. M. Hill 14. Automation in DNA sequencing Alan T. Bankier 15. Sequence databases and the internet Anne E. Hughes Introduction to DNA Sequencing. M13 Cloning. M13 Sequencing. Primer Design and Primer Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene Shotgun Sequencing. Cycle Sequencing. Cycle Sequencing of PCR-Amplified Genomic DNA With Dye-Labelled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyser. Fluorescent Sequencing Protocols for the ALF Fluorescent Sequencing for Heterozygote MutationDetection. Automation in DNA Sequencing. Sequence Databases and the Internet.