Conformation of Biological Molecules
New Results from NMR
Published on 7. December 2011
Book
Paperback/Softback
VIII, 218 pages
978-3-642-68099-1 (ISBN)
Description
The determination of the three-dimensional structure of a biological molecule is the starting point in the understanding of molecular mechanisms involved in its complex biochemical reactions. The molecular architecture of multimolecular systems such as membranes and chromosomes provides the key to the fascinating field of molecular biology. Stereochemical details of biological macromolecules and their interactions with pharmacological agents form the basis for drug design. Naturally, the study of the structure and function of biological molecules has aroused tremendous interest and investigations in this area are being carried out in a large number of laboratories. The techniques used for this purpose include both experimental methods (X-ray and neutron diffraction measurements, study of NMR, ESR, vibrational and electronic spectra, ORD, CD and dipole moment measurements, biochemical modifications etc. ) and the oretical methods (quantum mechanical and classical potential energy calculations, Monte Carlo simulations and molecular graphics). F or several years now, X-ray diffraction [1] has served as our only source of infor mation on the three-dimensional arrangements of atoms in biopolymers. Fiber-diffrac tion of DNA led to the proposal of the DNA double helix. Fibers of long~hain polymers show ordering in the direction of the fibre-axis but not in the transverse plane. Accurate estimates of the dimensions of helical structures can be made using techniques on the basis of which models of biopolymers can be constructed.
More details
Series
Edition
Softcover reprint of the original 1st ed. 1982
Language
English
Place of publication
Berlin
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Research
Illustrations
VIII, 218 p.
Dimensions
Height: 244 mm
Width: 170 mm
Thickness: 13 mm
Weight
409 gr
ISBN-13
978-3-642-68099-1 (9783642680991)
DOI
10.1007/978-3-642-68097-7
Schweitzer Classification
Other editions
Additional editions
E-Book
12/2012
Springer
€96.29
Available for download
Book
11/1981
Springer
€85.55
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Content
1 General Theory.- 1.1 Introduction.- 1.2 What is Conformation?.- 1.3 Conformational Theory.- 1.4 Structure of Long-Chain Polymers.- 1.5 Problems in NMR Studies of Biological Molecules.- 2 NMR Techniques in Conformational Studies.- 2.1 Coupling Constants.- 2.2 Chemical Shifts of Hydrogen-Bonded Protons.- 2.3 Magnetic Anisotropy of Chemical Bonds or Groups.- 2.4 13C Chemical Shifts.- 2.5 31P Chemical Shifts.- 2.6 Relaxation Times (T1 and T2).- 2.7 Nuclear Overhauser Effect.- 2.8 Paramagnetic Reagents.- 2.9 Use of Liquid Crystals as Solvents.- 2.10 Deuterium Quadrupole Coupling Constants.- 2.11 Solvent Accessibility.- 2.12 From NMR Parameters to Spatial Structures.- 3 Nucleosides, Nucleotides and Nucleic Acids.- 3.1 Description of Nucleotide Structures.- 3.2 Glycosidic Bond Rotations.- 3.3 Sugar Ring Conformation: Pseudorotation.- 3.4 Backbone Angles.- 3.5 NMR Studies on Nucleosides.- 3.6 Small Nucleotides.- 3.7 Dinucleoside Phosphates and Short Segments of Nucleic Acids.- 3.8 Random-Coil Polynucleotides.- 3.9 Helical Polynucleotides.- 3.10 t-RNA.- 3.11 Drug-Nucleic Acid Interactions.- 4 Amino Acids, Peptides and Proteins.- 4.1 Description of the Structure of Peptide Units.- 4.2 Theoretical Considerations. The ΓΈ, ? Maps.- 4.3 NMR Techniques in the Study of Peptide Conformations.- 4.4 Conformations of Amino Acids.- 4.5 Linear Peptides.- 4.6 Cyclic Peptides.- 4.7 Homopolymeric Peptides. Helix-Coil Transition.- 4.8 Characterization of Protein Structures by NMR.- 4.9 Protein-Nucleic Acid Interaction.- 5 Polysaccharides.- 5.1 Structures of Polysaccharides and Carbohydrates.- 5.2 Conformations of Monosaccharides.- 5.3 Conformations of Polysaccharides.- 5.4 Glycoproteins and Peptide-Carbohydrate Interactions.- 6 Lipids and Molecular Organization in Membranes.- 6.1Biomembranes.- 6.2 General Properties of Phospholipids.- 6.3 Conformations of Phospholipids.- 6.4 Membrane Organization and Fluidity.- 6.5 Lipid-Protein and Lipid-Cholesterol Interactions.- 7 Acknowledgements.- 8 References.- 9 Appendix.- 10 Subject Index.