Hplc Of Biological Macro- Molecules, Revised And Expanded
2nd Edition
Published on 8. January 2002
Book
Hardback
792 pages
978-0-8247-0665-4 (ISBN)
Description
Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
More details
Edition
2nd edition
Language
English
Place of publication
Bosa Roca
United States
Publishing group
Taylor & Francis Inc
Target group
College/higher education
Professional and scholarly
Dimensions
Height: 235 mm
Width: 157 mm
Thickness: 47 mm
Weight
1276 gr
ISBN-13
978-0-8247-0665-4 (9780824706654)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Karen M. Gooding | Fred E. Regnier
Hplc Of Biological Macro- Molecules, Revised And Expanded
E-Book
01/2002
2nd Edition
CRC Press
€456.99
Available for download
Karen M. Gooding | Fred E. Regnier
Hplc Of Biological Macro- Molecules, Revised And Expanded
E-Book
01/2002
2nd Edition
CRC Press
€456.99
Available for download
Persons
Gooding, Karen M.; Regnier, Fred E.
Content
THE TECHNIQUE. Silica as a Support in HPLC of Biomacromolecules. Organic Polymer Support Materials. Size Exclusion Chromatography. Ion-Exchange Chromatography. Reversed Phase and Hydrophobic Interaction Chromatography of Proteins and Peptides. Metal Interaction Chromatography. Preparative Chromatography. Sample Preparation. Gradient Elution Separation of Large Biomolecules. CLASS SPECIFIC APPLICATIONS. Analytical HPLC of Peptides. HPLC of Membrane Proteins. HPLC of Cereal Endosperm Storage Proteins. Analysis of Hemoglobin Variants and HbA1c, U. Turpeinen. Antibodies. Glycoproteins. DETECTION METHODS. Immunodetection of Proteins in High-Resolution Separation Systems. Characterization of Proteins, Peptides, and Polynucleotides by Mass Spectrometry. Detection and Analysis of Proteins by High Performance Liquid Chromatography with Photodiode Array Detection. Index.