DNA Cloning
2nd Edition
Published on 1. May 1995
Book
Paperback/Softback
273 pages
978-0-19-963479-8 (ISBN)
Description
Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. Part of a four-volume set on DNA cloning, this book covers the main expression systems in current use. It provides background information and advice on the merits of each, step-by-step practical protocols, and details of the latest applications.
More details
Edition
2nd Revised edition
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Edition type
Revised edition
Illustrations
halftones, line figures, tables, bibliography
ISBN-13
978-0-19-963479-8 (9780199634798)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Screening expression libraries with antibody and protein probes; Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies; Purification of over-produced proteins from E.coli cells; Production of monoclonal antibodies against proteins expressed in E.coli; Expression of cloned genes in yeast; Intraction trap cloning with yeast; The baculovirus expression system.