
DNA Cloning 4: A Practical Approach
Mammalian Systems
Oxford University Press
Published on 25. January 1996
Book
Paperback/Softback
340 pages
978-0-19-963480-4 (ISBN)
Description
DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the
HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and
troubleshooting comments.
This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.
HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and
troubleshooting comments.
This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.
More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Illustrations
halftones, line figures, tables
Dimensions
Height: 234 mm
Width: 157 mm
Thickness: 17 mm
Weight
514 gr
ISBN-13
978-0-19-963480-4 (9780199634804)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Editor
Department of Anatomy and PhysiologyUniversity of Dundee
Department of Biochemistry and Molecular BiologyUniversity of Leeds
Content
1. High efficiency gene transfer into mammalian cells ; 2. Construction and characterization of Vaccinia virus recombinants ; 3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells ; 4. Retroviral vectors ; 5. Genetic manipulation of embryonic stem cells ; 6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs ; 7. Genomic and expression analysis of transgenic animals ; 8. Expression using a defective Herpes simplex virus (HSV-1) vector system ; 9. Adenovirus vectors