DNA Cloning 2: A Practical Approach
Expression Systems
2nd Edition
Published on 11. May 1995
Book
Paperback/Softback
274 pages
978-0-19-963478-1 (ISBN)
Description
Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. This book covers all the main expression systems in current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
Reviews / Votes
a worthwhile bench top companion * Henry Brzeski, University of Strathclyde, Biochemical Systematics and Ecology, Vol. 24, No. 1, 1996 * The present book consists of 7 chapters that each coprehensively describe different aspects of the subject and which may be read independently ... this book is very detailed and informative and contains much information with key literature citations of use for any scientist working in the area of heterologous protein expression ... The book can thus be highly recommended. * Bent Honore, FEBS Letters 393 (1996) *More details
Series
Edition
2nd Revised edition
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Edition type
Revised edition
Illustrations
halftones, line figures, tables
Dimensions
Height: 234 mm
Width: 156 mm
Thickness: 15 mm
Weight
424 gr
ISBN-13
978-0-19-963478-1 (9780199634781)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Content
1. Screening expression libraries with antibody and protein probes ; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies ; 3. Purification of over-produced proteins from E.coli cells ; 4. Production of monoclonal antibodies against proteins expressed in E.coli ; 5. Expression of cloned genes in yeast ; 6. Intraction trap cloning with yeast ; 7. The baculovirus expression system