
Flow Cytometry
First Principles
Wiley (Publisher)
3rd Edition
Will be published approx. on 16. April 2026
Book
Paperback/Softback
352 pages
978-1-119-50811-3 (ISBN)
Description
A valuable resource for introducing flow cytometry, providing a foundation for understanding its importance in various fields
The newly updated and revised third Edition of Flow Cytometry: First Principles, 3rd Edition covers flow cytometric theory and the fundamentals of instrumentation and data analysis using examples from a wide range of scientific and clinical applications. Readers will find and learn about both standard and advanced flow cytometric instrumentation, methods for analyzing flow data, and applications of the technique ranging from immunology, clinical diagnoses, DNA analysis, and cell sorting, as well as novel applications such as microbiology, aquatic biology, and molecular biology. Each chapter starts with basic knowledge and gradually expands on the subject matter, enabling readers to assess the ways that flow cytometry has been used in their respective fields and the ways that it might be applied in the future. A selected number of annotated references are given, aiming to point readers into relevant literature with an extensive glossary at the end.
Written by two experts and highly illustrated, Flow Cytometry: First Principles, Third Edition includes information on:
How flow cytometry is used to analyze cells
How a flow cytometer can be used to separate different types of cells from each other
Fluidics, electronics, and optics (including lasers, fluorochromes, mirrors, and filters)
Clinical applications of flow cytometry
An indispensable authoritative text on flow cytometry, the third edition of Flow Cytometry: First Principles is an essential resource for scientists, clinicians, technologists, instructors, and students.
The newly updated and revised third Edition of Flow Cytometry: First Principles, 3rd Edition covers flow cytometric theory and the fundamentals of instrumentation and data analysis using examples from a wide range of scientific and clinical applications. Readers will find and learn about both standard and advanced flow cytometric instrumentation, methods for analyzing flow data, and applications of the technique ranging from immunology, clinical diagnoses, DNA analysis, and cell sorting, as well as novel applications such as microbiology, aquatic biology, and molecular biology. Each chapter starts with basic knowledge and gradually expands on the subject matter, enabling readers to assess the ways that flow cytometry has been used in their respective fields and the ways that it might be applied in the future. A selected number of annotated references are given, aiming to point readers into relevant literature with an extensive glossary at the end.
Written by two experts and highly illustrated, Flow Cytometry: First Principles, Third Edition includes information on:
How flow cytometry is used to analyze cells
How a flow cytometer can be used to separate different types of cells from each other
Fluidics, electronics, and optics (including lasers, fluorochromes, mirrors, and filters)
Clinical applications of flow cytometry
An indispensable authoritative text on flow cytometry, the third edition of Flow Cytometry: First Principles is an essential resource for scientists, clinicians, technologists, instructors, and students.
More details
Edition
3rd edition
Language
English
Place of publication
Hoboken
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Dimensions
Height: 228 mm
Width: 154 mm
Thickness: 20 mm
Weight
544 gr
ISBN-13
978-1-119-50811-3 (9781119508113)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions

E-Book
03/2026
3rd Edition
Wiley
€81.99
Available for download

E-Book
03/2026
3rd Edition
Wiley
€81.99
Available for download
Previous edition

Book
07/2001
2nd Edition
Wiley
€94.00
Shipment within 10-20 days
Persons
Alice Longobardi Givan received her A.B. at Bryn Mawr College and her Ph.D. at Harvard University. She was Assistant Director / Director of the Flow Cytometry Core Laboratory in the Norris Cotton Cancer Center at Dartmouth Medical School, Lebanon, NH, from 1991-2010.
Paul K. Wallace received his B.S. from The New England College, his M.S. from Idaho State University, and his Ph.D. from Drexel University. He was President of the International Society for Advancement of Cytometry (ISAC). He was Director of the Department of Flow and Image Cytometry, Roswell Park Comprehensive Cancer Center, Buffalo, NY. Currently, he is the CSO of SciGro, Inc., Sedona, AZ.
Paul K. Wallace received his B.S. from The New England College, his M.S. from Idaho State University, and his Ph.D. from Drexel University. He was President of the International Society for Advancement of Cytometry (ISAC). He was Director of the Department of Flow and Image Cytometry, Roswell Park Comprehensive Cancer Center, Buffalo, NY. Currently, he is the CSO of SciGro, Inc., Sedona, AZ.
Author
Bryn Mawr College; Harvard University
New England College; Idaho State University; Drexel University
Illustrated by
Content
The Past as Prologue
Setting the Scene
Instrumentation: Into the Black Box
Information: Harnessing the Data
Seeing the Light: Lasers, Fluorochromes, Mirrors, Prisms, and Filters
Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating
Cells from Within: Intracellular Proteins
Cells from Within: DNA in Life and Death
Assays of Function
The Sorting of Cells
Disease and Diagnosis: The Clinical Laboratory
Out of the Mainstream: Biological Frontiers
Out of the Mainstream" Technological Frontiers
Flowing On: The Future
Selected General Resources
Glossary
Setting the Scene
Instrumentation: Into the Black Box
Information: Harnessing the Data
Seeing the Light: Lasers, Fluorochromes, Mirrors, Prisms, and Filters
Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating
Cells from Within: Intracellular Proteins
Cells from Within: DNA in Life and Death
Assays of Function
The Sorting of Cells
Disease and Diagnosis: The Clinical Laboratory
Out of the Mainstream: Biological Frontiers
Out of the Mainstream" Technological Frontiers
Flowing On: The Future
Selected General Resources
Glossary