DNA and RNA Isolation Techniques for Non-Experts
Published on 30. March 2022
Book
Hardback
XVI, 203 pages
978-3-030-94229-8 (ISBN)
Description
This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
More details
Series
Edition
2022 ed.
Language
English
Place of publication
Cham
Switzerland
Publishing group
Springer International Publishing
Target group
Professional and scholarly
Illustrations
48 farbige Abbildungen, 10 s/w Abbildungen
XVI, 203 p. 58 illus., 48 illus. in color.
Dimensions
Height: 241 mm
Width: 160 mm
Thickness: 18 mm
Weight
500 gr
ISBN-13
978-3-030-94229-8 (9783030942298)
DOI
10.1007/978-3-030-94230-4
Schweitzer Classification
Other editions
Additional editions
Akash Gautam
DNA and RNA Isolation Techniques for Non-Experts
Book
03/2023
Springer
€90.94
Shipment within 7-9 days
Akash Gautam
DNA and RNA Isolation Techniques for Non-Experts
E-Book
03/2022
1st Edition
Springer
€90.94
Available for download
Person
Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).
Content
1-Basic Idea About Nucleic Acids For Non-Experts.- 2-General Steps During Isolation Of DNA And RNA.- 3-Phenol-Chloroform DNA Isolation Method.- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method.- 5-Spin Column-Based Isolation Of Nucleic Acid.- 6-Isolation Of Plasmid DNA By Alkaline Lysis.- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment.- 8-Lithium Chloride Based Isolation Of DNA And RNA.- 9-Isolation Of Bacteriophage DNA By Peg Method.- 10-DNA Isolation By Chelex Method.- 11-DNA Isolation By Lysozyme And Proteinase K.- 12-Salting-Out Method For Blood Samples.- 13-CTAB Or SDA Based Isolation Of Plant's DNA.- 14-DNA Extraction By Spooling Method.- 15-Magnetic Bead-Based Nucleic Acid Isolation.- 16-Density Gradient-Based Nucleic Acid Isolation.- 17-DNA Extraction From Agarose Gel Though Paper-Strips.- 18-Transformation Or Genetic Modification Of Cells/Organisms.- 19-Gene Cloning And Vectors.- 20-Polymerase Chain Reaction (PCR).- 21-Southern And Northern Blotting.- 22-Genome Mapping.- 23-Applications Of DNA Sequencing Technologies For Current Research.