A Laboratory Guide to the Mammalian Embryo

Preface; Acknowledgements; List of Contributors; Abbreviations; 1. Follicle development in vitro; 2. Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery; 3. Culture of the mammalian preimplantation embryo; 4. Assessment of embryo development and viability; 5. Micromanipulation : ICSI; 6. Micromanipulation : Biopsy; 7. Analysis of intracellular ions: pH and calcium; 8. Nutrient uptake and metabolite production and enzyme activity and regulation; 9. Metabolic Pathway; 10. Confocal imaging of structural molecules in mammalian gametes; 11. Fluorescent in situ hybridization; 12. Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos; 13. Microscale RNA isolation from mammalian embryos; 14. Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR); 15. Characterization of novel genes during early development by application of differential display RT-PCR; 16. Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells; 17. Antisense disruption of gene expression in the preimplantation embryo; 18. Gene subtraction and analysis; 19. Analysis of apoptosis in the preimplantation embryo; 20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo; 21. Human Embryonic Stem; 22. Spermatogonial Transplantion; 23. Somatic Cell Nuclear Transfer; 24. Cryobiology; slow freezing vitrification of embryos