A Practical Introduction to DNA Fingerprinting
D.A. Galbraith(Author)
Chapman and Hall (Publisher)
Published in November 1996
Book
Hardback
224 pages
978-0-412-43410-5 (ISBN)
Description
This is a practical guide to the use of DNA fingerprinting and related techniques of molecular genetics for applications in the biological sciences. It covers the theoretical background, planning, methodology, implementation, and analysis of DNA profiles for applications such as paternity analysis and analysis of relatedness in field populations of vertebrates. Written especially for scientists with no background in molecular genetics but who are interested in wildlife biology, conservation, demographics and evolutionary and behavioural ecology, this book bridges the gap between the specialized molecular techniques and their practical applications in ecological and evolutionary studies. It is a step-by-step guide combining training exercises, "hands-on" protocols, and detailed background information to give the researcher a guided introduction to the actual use of DNA fingerprinting in her own applications. This book should be of interest to wildlife biologists; conservation biologists; ecologists; population biologists; geneticists and other molecular biologists; and forensic scientists.
More details
Language
English
Place of publication
London
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
45 illustrations, glossary, appendices, references, index
Dimensions
Height: 234 mm
Width: 156 mm
Weight
333 gr
ISBN-13
978-0-412-43410-5 (9780412434105)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
The history and theory of DNA fingerprinting. Elements of experimental design for DNA fingerprinting. Genomes, genetics, and molecular methods. The technique of DNA fingerprinting. Laboratory safety. Fundamental laboratory skills. Organizing and equipping the laboratory. Laboratory protocols for DNA fingerprinting. Introduction to the protocol chapters. A lambdaDNA model for southern blots. Extraction and preparation of nuclear DNA. Agarose gel electrophoresis. Southern blotting. Solid base DNA hybridization of labelled probes. Interpretation and scoring of autoradiograms. Analysis of DNA fingerprint data. Epiolgue: whither DNA fingerprinting?