Proteinase Inhibitors

Proteinase Inhibitors.- A Turbulent Life.- Welcome.- Section I Plasma Proteinase Inhibitors.- Biochemistry of Proteinase Inhibitors from Human Plasma: A Review of Recent Development.- Purification, Properties, and Composition of ?1-Trypsin Inhibitor from Human Plasma.- Properties of Human ?1-Antitrypsin.- Human ?1-Antitrypsin Phenotypes.- Isolation and Fragmentation of ?1-Antitrypsin.- The Interaction of Human Cationic Trypsin and Chymotrypsin II with Human Serum Inhibitors.- Studies on the Quaternary Structure of Human Serum ?2-Macroglobulin.- The Unique Nature of the Interaction of ?2-Macroglobulin with Proteinases.- Biology and Pathology of Plasma Proteinase Inhibitors.- Interaction between Endogenous Proteases and Plasma Protease Inhibitors in vitro and in vivo.- Plasma Clearance of Human Antiproteinase/Proteinase Complexes.- Protease Inhibitors in Human Synovial Fluids of Patients with Joint Diseases.- Proteinase Inhibitors of the Respiratory Tract: Studies on the Structural Relationship between Acid-Stable Inhibitors Present in the Respiratory Tract, Plasma and Urine.- Section II Proteinases and Proteinase Inhibitors in Fertilization.- Biochemistry and Clinical Significance of the Trypsin-Like Proteinase Acrosin from Boar and Human Spermatozoa.- Studies on the Purification and Characterization of Boar Acrosin.- Proteinase Inhibitors and Proteinases of Human Semen.- Characterization of Two Proteinase Inhibitors from Human Seminal Plasma and Spermatozoa.- Isolation of Proteinase Inhibitors from Boar Sperm Acrosomes and Boar Seminal Plasma and Effect on Fertilization.- Purification,Characterization,and Structural Studies of Proteinase Inhibitors from Boar Seminal Plasma and Boar Spermatozoa.- Proteinase Inhibitors in Human Cervical Mucus and Their in vitro Interactions with Human Acrosin.- Section III Pancreatic Proteinase Inhibitors.- Human Pancreatic Secretory Trypsin Inhibitor.- Isolation of Human Pancreatic Inhibitor and the Study of Its Interaction with Mammalian and Human Proteases.- Studies on Inhibition of the Two Human Trypsins.- Sequence of Cleavages in Temporary Inhibitors: Porcine Inhibitor Form II and Bovine Isoinhibitors A and C.- Temporary Inhibition - A Result of Consecutive Peptide Bond Hydrolysis.- Measurement of the Bovine Pancreatic Trypsin Inhibitors by Radioimmunoassay.- Properties and Inhibition Spectrum of a New Human Pancreatic Protease.- Section IV Characterization and Structure of New Inhibitors.- Amino Acid Sequence of Snail Inhibitor K and Correlation of Structure and Specificity.- Novel Proteinase Inhibitors in Snake Venoms: Distribution, Isolation, and Amino Acid Sequence.- Broad Specificity Inhibitors from Sea Anemones.- Discussion Remark: A Polyvalent Proteinase Inhibitor in the Earthworm (Lumbricus terrestris).- Discussion Remark: Proteinase Isoinhibitors from Cuttle Fish (Loligo vul-garis) H.Tschesche.- The Partial Linear Sequence of Garden Bean Inhibitor II and Location of the Protease Reactive Sites.- Elastase Inhibitors as Impurities in Commercial Preparations of Soybean Trypsin Inhibitor (Kunitz).- A New Alkaline Proteinase Inhibitor from Streptomyces albogriseolus.- Section V Structure-Function Relationships.- Replacements, Insertions, and Modifications of Amino Acid Residues in the Reactive Site of Soybean Trypsin Inhibitor (Kunitz).- Chemical Structure and Inhibitory Activities of Soybean Proteinase Inhibitors.- Structure-Function Relationships in Lima Bean Protease Inhibitor.- Structure-Activity Relationships of Several Trypsin and Chymotrypsin Inhibitors from Legume Seeds.- Reactive Site Cleavage, Thermodynamic Control Resynthesis, and Properties of Chemically Derivatized Trypsin-Kallikrein Inhibitors.- The Interaction of Catalytically Inactive Proteolytic Enzymes with Protein Inhibitors. Methyl-Chymotrypsin.- Discussion Remark: Inhibition of Anhydro-Trypsin and Anhydro- Chymotrypsin.- Section VI Physical-Chemical Studies of Enzyme-Inhibitor Interactions.- Kinetics and Thermodynamics of the Interaction of Proteinases with Protein Inhibitors.- Kinetic Studies of the Binding of Bovine Basic Pancreatic Trypsin Inhibitor to ?-Chymotrypsin.- The Mechanism of Association of Trypsin (or Chymotrypsin) with the Pancreatic Trypsin Inhibitors (Kunitz and Kazal), Kinetics and Thermodynamics of the Interaction.- Kinetic Studies on the Interactions between Native, Acetylated and Succinylated Trypsin and Natural Proteinase Inhibitors.- The Use of Fluorescence Techniques in the Study of the Interaction of the Basic Trypsin Inhibitor of Bovine Pancreas, Selectively Labelled at Lysine 15, with Chymotrypsin and Trypsin.- Raman Studies of Native and Partially (Cys 14-Cys 38) Reduced Basic Pancreatic Trypsin Inhibitor.- A Study on the Interaction of Trasylol with Trypsin and Kallikrein by Circular Dichroism.- Some Kinetic Consequences of the Tight Binding of Protein-Proteinase- Inhibitors to Proteolytic Enzymes and Their Application to the Determination of Dissociation Constants.- Section VII Molecular Aspects of Enzyme-Inhibitor Interactions.- Stereochemistry of Substrate Binding and Hydrolysis in the Trypsin Family of Enzymes.- The Model of the Basic Pancreatic Trypsin Inhibitor Refined at 1.5 A Resolution.- Structure of the Complex Formed by Bovine Trypsin and Bovine Pancreatic Trypsin Inhibitor, Refinement of the Crystal Structure Analysis.- The Mode of Action of Soybean Trypsin Inhibitor as Revealed by Crystal Structure Analysis of the Complex with Porcine Trypsin.- Section VIII Organic Chemical Models and Low-Molecular-Weight Inhibitors.- Organic Chemical Models for Proteinase Inhibitors.- Progress in Designing Small Inhibitors Which Discriminate among Trypsin-Like Enzymes.- Homoarginine Oligomers as Trypsin and Chymotrypsin Substrates and Inhibitors.- Proteinase Inhibitors of Microbial Origin. A Review.- Discussion Remark: Low Molecular Weight Carboxydipeptidase Inhibitors from Bothropsjararaca Venom.- Section IX Inhibition of Non-Serine Proteases.- Carboxypeptidase Inhibitor from Potatoes. Properties and Amino Acid Sequence.- Inhibitors of Lysosomal Proteinases.- Inhibition of Insulin Specific Protease by Proteinase Inhibitors from the Snail (Helix pomatia).- An Insulin Degrading Proteinase from Human Erythrocytes and Its Inhibition by Proteinase Inhibitors.- Section X Evolution of Specificity of Proteinase Inhibitors.- Evolution of Specificity of Protein Proteinase Inhibitors.- Section XI Proteinases and Inhibitors in Biological Systems.- Proteases and Protease Inhibitors in Neoplasia.- Secretion of Enzymes by Neoplastic Cells and Macrophages.- Plasminogen Activators of Normal and Transformed Cells.- Proteinase Inhibitors from Ascaris lumbricoides: Properties and Their Physiological Role.- Wound-Induced Accumulation of Proteinase Inhibitor I in Tomato Leaves.- Section XII General Discussion Remark, Resume, Concluding Remarks.- General Discussion Remark: Energetics of Inhibitor Binding.- Resume.- Concluding Remarks.- Author Index.