Drug'DNA Interaction Protocols
Methods, Case Studies and Protocols
Published on 7. October 1997
Book
Hardback
X, 278 pages
978-0-89603-447-1 (ISBN)
Description
A collection of useful molecular techniques to illuminate and explore the interaction of drugs and ligands with DNA. These easily reproducible methods involve sequence recognition properties, as well as the physical approaches for measuring both the strength of interaction and the mode of drug binding to DNA. The interactions are also examined from a cellular perspective and for their usefulness in the design of new therapeutic agents. The powerful techniques detailed here will be particularly useful in elucidating the action of existing therapeutic agents, as well as in the design of new anti-cancer drugs with improved action and reduced toxicity.
Reviews / Votes
Methods in Molecular Biology is a long standing, respected series that provides protocols for the researcher. ..this particular book is a welcome addition for the researcher. It is recommended for all medical and science libraries. - Reviewer: H. Robert Malinowsky, E-STREAMSMore details
Series
Edition
1997. Corr. 3rd Printing ed.
Language
English
Place of publication
NJ
United States
Target group
College/higher education
Professional and scholarly
Illustrations
1
Dimensions
Height: 22.9 cm
Width: 15.2 cm
Thickness: 20 mm
Weight
575 gr
ISBN-13
978-0-89603-447-1 (9780896034471)
DOI
10.1385/089603447X
Schweitzer Classification
Other editions
New editions
Keith R. Fox
Drug-DNA Interaction Protocols
Book
12/2009
2nd Edition
Humana Press Inc.
€106.99
Shipment within 15-20 days
Content
DNase I Footprinting
Keith R. Fox
Quantitative DNA Footprinting
James C. Dabrowiak, Jerry Goodisman, and Brian Ward
Uranyl Photoprobing of DNA Structures and Drug-DNA Complexes
Niels Erik Møllegard and Peter E. Nielsen
Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro
Christian Bailly and Michael J. Waring
Footprinting Studies with Nucleosome-Bound DNA
Philip M. Brown and Keith R. Fox
A Gel Mobility Shift Assay for Probing the Effect of Drug-DNA Adducts on DNA-Binding Proteins
Suzanne M. Cutts, Andrew Masta, Con Panousis, Peter G. Parsons, Richard A. Sturm, and Don R. Phillips
An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug-Binding Sites
Suzanne M. Cutts, Con Panousis, Andrew Masta, and Don R. Phillips
DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug-DNA Interactions
Thomas P. Dooley and Katherine L. Weiland
Transcriptional Footprinting of Drug-DNA Interactions
Don R. Phillips and Carleen Cullinane
Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays
John A. Hartley and Michael D. Wyatt
PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells
Keith A. Grimaldi and John A. Hartley
Analysis of DNA-Binding Antibodies
Jeremy S. Lee, Laura J. P. Latimer, and Jamshid Tanha
Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies
Jeremy S. Lee, Laura J. P. Latimer, and Gary D. Burkholder
Optical Absorbance and Fluorescence Techniques for Measuring DNA-Drug Interactions
Terence C. Jenkins
Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves
W. David Wilson, Farial A. Tanious, Maria Fernandez-Saiz, and C. TedRigl
Electric Dichroism
Dietmar Porschke
Calorimetric Techniques for Studying Drug-DNA Interactions
Harry P. Hopkins, Jr
Methods for the Studies of Drug Dissociation from DNA
Fu-Ming Chen
Index
Keith R. Fox
Quantitative DNA Footprinting
James C. Dabrowiak, Jerry Goodisman, and Brian Ward
Uranyl Photoprobing of DNA Structures and Drug-DNA Complexes
Niels Erik Møllegard and Peter E. Nielsen
Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro
Christian Bailly and Michael J. Waring
Footprinting Studies with Nucleosome-Bound DNA
Philip M. Brown and Keith R. Fox
A Gel Mobility Shift Assay for Probing the Effect of Drug-DNA Adducts on DNA-Binding Proteins
Suzanne M. Cutts, Andrew Masta, Con Panousis, Peter G. Parsons, Richard A. Sturm, and Don R. Phillips
An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug-Binding Sites
Suzanne M. Cutts, Con Panousis, Andrew Masta, and Don R. Phillips
DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug-DNA Interactions
Thomas P. Dooley and Katherine L. Weiland
Transcriptional Footprinting of Drug-DNA Interactions
Don R. Phillips and Carleen Cullinane
Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays
John A. Hartley and Michael D. Wyatt
PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells
Keith A. Grimaldi and John A. Hartley
Analysis of DNA-Binding Antibodies
Jeremy S. Lee, Laura J. P. Latimer, and Jamshid Tanha
Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies
Jeremy S. Lee, Laura J. P. Latimer, and Gary D. Burkholder
Optical Absorbance and Fluorescence Techniques for Measuring DNA-Drug Interactions
Terence C. Jenkins
Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves
W. David Wilson, Farial A. Tanious, Maria Fernandez-Saiz, and C. TedRigl
Electric Dichroism
Dietmar Porschke
Calorimetric Techniques for Studying Drug-DNA Interactions
Harry P. Hopkins, Jr
Methods for the Studies of Drug Dissociation from DNA
Fu-Ming Chen
Index